Rap1P and Telomere Length Regulation in Yeast

  1. Derek J. Chadwick Organizer and
  2. Gail Cardew Organizer
  1. Stéphane Marcand1,2,
  2. David Wotton1,
  3. Eric Gilson2 and
  4. David Shore1,3,†

Published Online: 28 SEP 2007

DOI: 10.1002/9780470515433.ch6

Ciba Foundation Symposium 211 - Telomeres and Telomerase

Ciba Foundation Symposium 211 - Telomeres and Telomerase

How to Cite

Marcand, S., Wotton, D., Gilson, E. and Shore, D. (2007) Rap1P and Telomere Length Regulation in Yeast, in Ciba Foundation Symposium 211 - Telomeres and Telomerase (eds D. J. Chadwick and G. Cardew), John Wiley & Sons, Ltd., Chichester, UK. doi: 10.1002/9780470515433.ch6

Author Information

  1. 1

    Department of Microbiology, College of Physicians & Surgeons of Columbia University, 701 West 168th Street, NY 10032, USA

  2. 2

    Ecole Nor male Super ieure de Lyon, UMR49 CNRS/ENSE, 69364 Lyon Cédex 07, France

  3. 3

    Department of Molecular Biology, University of Geneva, Sciences II, 30 quai Ernest-Ansermet, CH-1211 Geneva 4, Switzerland

  1. This chapter was presented at this symposium by David Shore. Present address: Department of Molecular Biology, University of Geneva.

Publication History

  1. Published Online: 28 SEP 2007

ISBN Information

Print ISBN: 9780471972785

Online ISBN: 9780470515433

SEARCH

Keywords:

  • rap1p length regulation;
  • telomere length regulation;
  • yeast;
  • histone-binding silencer proteins;
  • RAP1P C-termini

Summary

Telomere length in the yeast Saccbaroyces cereuisiae is under stringent genetic control such that a narrow length distribution of TG1–3 repeats is observed. Previous studies have shown that Rap1p, which binds to the double-stranded telomeric repeats, plays a role in regulating repeat length: point mutations in the Rap1p C-terminus often result in a higher average telomere length and deletion of this region causes extreme telomere elongation. We have investigated further the role of Rap1p in this process. Our results suggest that telomere length is regulated by a negative feedback mechanism that can sense the number of Rap1p molecules bound at the chromosome end. This length regulatory mechanism requires two other proteins, Rif1p and Rif2p, that interact with each other and with the Rap1p C-terminus. Although the same C-terminal domain of Rap1p is also involvcd in the initiation of telomere position effect (telomeric transcriptional silencing), this Rap1p function appears to be separate from, and indeed antagonistic to, its role in telomere length regulation.