Chapter 12. Immunoassays and Enzyme-Based Assays

  1. Robert J. Flanagan1,
  2. Andrew Taylor2,
  3. Ian D. Watson3,
  4. Robin Whelpton4

Published Online: 1 FEB 2008

DOI: 10.1002/9780470516294.ch12

Book Title

Fundamentals of Analytical Toxicology

Fundamentals of Analytical Toxicology

Additional Information

How to Cite

Flanagan, R. J., Taylor, A., Watson, I. D. and Whelpton, R. (2008) Immunoassays and Enzyme-Based Assays, in Fundamentals of Analytical Toxicology, John Wiley & Sons, Ltd, Chichester, UK. doi: 10.1002/9780470516294.ch12

Author Information

  1. 1

    Department of Clinical Biochemistry, King's College Hospital NHS Foundation Trust, London, UK

  2. 2

    Royal Surrey County Hospital, Guildford, Surrey, UK

  3. 3

    Department of Clinical Biochemistry, University Hospital Aintree, Liverpool, UK

  4. 4

    School of Biological and Chemical Sciences, Queen Mary, University of London, London, UK

Publication History

  1. Published Online: 1 FEB 2008
  2. Published Print: 25 JAN 2008

ISBN Information

Print ISBN: 9780470319345

Online ISBN: 9780470516294

SEARCH

CHAPTER TOOLS

Get PDF (295K)

Keywords:

  • serum protein-binding assay;
  • antibody-bound and nonbound label;
  • insensitive turbidimetric assay;
  • clinical chemistry analyzers;
  • antiinsulin or anti-proinsulin antibodies

Summary

This chapter contains sections titled:

  • Introduction

  • Basic principles of competitive binding assays

  • Heterogeneous immunoassays

  • Homogenous immunoassays

  • Microparticulate and turbidimetric immunoassays

  • Assay calibration, quality control and quality assurance

  • Interferences and assay failures

  • Enzyme-based assays

  • Summary

  • References

Get PDF (295K)