Target Identification by Diazirine Photo-Cross-Linking and Click Chemistry
Published Online: 1 DEC 2009
Copyright © 2009 by John Wiley and Sons, Inc.
Lab Protocol Title
Current Protocols in Chemical Biology
How to Cite
MacKinnon, A. L. and Taunton, J. 2009. Target Identification by Diazirine Photo-Cross-Linking and Click Chemistry. Current Protocols in Chemical Biology. 1:55–73.
- Published Online: 1 DEC 2009
Target identification is often the rate-determining step in deciphering the mechanism of action of biologically active small molecules. Photo-affinity labeling (PAL) represents a useful biochemical strategy for target identification in complex protein mixtures. This unit describes the use of alkyl diazirine-based photo-affinity probes and Cu(I)-catalyzed click chemistry to covalently label and visualize the targets of biologically active small molecules. A general method for affinity purification of probe-modified proteins, useful for identification of protein targets, is also described. Curr. Protoc. Chem Biol. 1:55-73. © 2009 by John Wiley & Sons, Inc.
- photo-affinity labeling;
- click chemistry;
- target identification;
- affinity purification