Unit

Nucleotide Analogues as Probes for DNA and RNA Polymerases

  1. Robert D. Kuchta

Published Online: 1 JUN 2010

DOI: 10.1002/9780470559277.ch090203

Current Protocols in Chemical Biology

Current Protocols in Chemical Biology

How to Cite

Kuchta, R. D. 2010. Nucleotide Analogues as Probes for DNA and RNA Polymerases. Current Protocols in Chemical Biology. 2:111–124.

Author Information

  1. University of Colorado, Boulder, Colorado

Publication History

  1. Published Online: 1 JUN 2010
  2. Published Print: JUN 2010

Abstract

Nucleotide analogues represent a major class of anti-cancer and anti-viral drugs, and provide an extremely powerful tool for dissecting the mechanisms of DNA and RNA polymerases. While the basic assays themselves are relatively straightforward, a key issue is to appropriately design the studies to answer the mechanistic question of interest. This unit addresses the major issues involved in designing these studies, and some of the potential difficulties that arise in interpreting the data. Examples are given for the type of analogues typically used, the experimental approaches with different polymerases, and issues with data interpretation. Curr. Protoc. Chem Biol. 2:111-124. © 2010 by John Wiley & Sons, Inc.

Keywords:

  • polymerase;
  • nucleotide;
  • DNA;
  • RNA;
  • kinetics