High-Throughput Assessment of Mammalian Cell Viability by Determination of Adenosine Triphosphate Levels
Published Online: 1 JUL 2010
Copyright © 2010 by John Wiley and Sons, Inc.
Lab Protocol Title
Current Protocols in Chemical Biology
How to Cite
Tolliday, N. 2010. High-Throughput Assessment of Mammalian Cell Viability by Determination of Adenosine Triphosphate Levels. Current Protocols in Chemical Biology. 2:153–161.
- Published Online: 1 JUL 2010
- Published Print: JUL 2010
There are many assays available for high-throughput assessment of mammalian cell viability or cytotoxicity. Approaches include measurement of metabolic capacity, intracellular adenosine triphosphate (ATP) levels, induction of apoptosis, intracellular esterase and protease activity, and cellular membrane integrity. This unit provides an in-depth protocol for measurement of cellular ATP levels as a readout of mammalian cell viability, using the CellTiter-Glo assay from Promega Corporation. A comparison of the key parameters (sensitivity, speed, and cost) for this and other common high-throughput viability assays is also presented. Curr. Protoc. Chem. Biol. 2:153-161 © 2010 by John Wiley & Sons, Inc.
- cell viability assay;
- cytotoxicity assay;
- high-throughput screening