Unit

High-Throughput Assessment of Mammalian Cell Viability by Determination of Adenosine Triphosphate Levels

  1. Nicola Tolliday

Published Online: 1 JUL 2010

DOI: 10.1002/9780470559277.ch100045

Current Protocols in Chemical Biology

Current Protocols in Chemical Biology

How to Cite

Tolliday, N. 2010. High-Throughput Assessment of Mammalian Cell Viability by Determination of Adenosine Triphosphate Levels. Current Protocols in Chemical Biology. 2:153–161.

Author Information

  1. Chemical Biology Platform, The Broad Institute of Harvard University and MIT, Cambridge, Massachusetts

Publication History

  1. Published Online: 1 JUL 2010
  2. Published Print: JUL 2010

Abstract

There are many assays available for high-throughput assessment of mammalian cell viability or cytotoxicity. Approaches include measurement of metabolic capacity, intracellular adenosine triphosphate (ATP) levels, induction of apoptosis, intracellular esterase and protease activity, and cellular membrane integrity. This unit provides an in-depth protocol for measurement of cellular ATP levels as a readout of mammalian cell viability, using the CellTiter-Glo assay from Promega Corporation. A comparison of the key parameters (sensitivity, speed, and cost) for this and other common high-throughput viability assays is also presented. Curr. Protoc. Chem. Biol. 2:153-161 © 2010 by John Wiley & Sons, Inc.

Keywords:

  • CellTiter-Glo;
  • cell viability assay;
  • cytotoxicity assay;
  • high-throughput screening