Unit

Supported Membrane Formation, Characterization, Functionalization, and Patterning for Application in Biological Science and Technology

  1. Wan-Chen Lin1,
  2. Cheng-Han Yu2,
  3. Sara Triffo1,
  4. Jay T. Groves1,2,3,4

Published Online: 1 DEC 2010

DOI: 10.1002/9780470559277.ch100131

Current Protocols in Chemical Biology

Current Protocols in Chemical Biology

How to Cite

Lin, W.-C., Yu, C.-H., Triffo, S. and Groves, J. T. 2010. Supported Membrane Formation, Characterization, Functionalization, and Patterning for Application in Biological Science and Technology. Current Protocols in Chemical Biology. 2:235–269.

Author Information

  1. 1

    Howard Hughes Medical Institute, Department of Chemistry, University of California, Berkeley, California

  2. 2

    Research Center of Excellence in Mechanobiology, National University of Singapore, Singapore

  3. 3

    Physical Biosciences Division, Lawrence Berkeley National Laboratory, Berkeley, California

  4. 4

    Materials Sciences Division, Lawrence Berkeley National Laboratory, Berkeley, California

Publication History

  1. Published Online: 1 DEC 2010
  2. Published Print: DEC 2010

Abstract

Supported membranes, formed as a single continuous lipid bilayer on a solid substrate, such as silica, have been used extensively as a model for protein-protein and cell-cell interaction, to study the molecular interactions at interfaces and the heterogeneities of plasma membranes. The advantages of a supported membrane system include the ability to control membrane composition and the compatibility it has with various surface-sensitive microscopic and spectroscopic techniques. Recent advances in micro- and nanotechnology have greatly extended the use of supported membranes to address key questions in cell biology. Although supported membranes can be easily made by vesicle fusion, the samples need careful preparation for this process to be efficient. The protocols in this unit comprehensively describe procedures to prepare, functionalize, and characterize supported membranes. Curr. Protoc. Chem. Biol. 2:235-269 © 2010 by John Wiley & Sons, Inc.

Keywords:

  • supported membrane;
  • supported lipid bilayer;
  • small unilamellar vesicle (SUV);
  • membrane functionalization;
  • fluorescence recovery after photobleaching (FRAP);
  • quantitative fluorescence measurement;
  • photolithography