Development of Chemical Probes for Biochemical Detection and Cellular Imaging of Myristoylated and Palmitoylated Proteins
Published Online: 1 FEB 2011
Copyright © 2011 by John Wiley and Sons, Inc.
Lab Protocol Title
Current Protocols in Chemical Biology
How to Cite
Hannoush, R. N. 2011. Development of Chemical Probes for Biochemical Detection and Cellular Imaging of Myristoylated and Palmitoylated Proteins. Current Protocols in Chemical Biology. 3:15–26.
- Published Online: 1 FEB 2011
- Published Print: FEB 2011
Fatty acylation of proteins regulates their spatial localization and activity in living cells. Methods to monitor fatty acylation are invaluable for studying its role in regulating protein dynamics. The protocols in this unit describe a procedure that involves metabolic labeling with ω-alkynyl fatty acids for detecting and cellular imaging of fatty-acylated proteins, namely myristoylated and palmitoylated proteins. Curr. Protoc. Chem. Biol. 3:15-26 © 2011 by John Wiley & Sons, Inc.
- alkynyl fatty acids;
- chemical probes;
- metabolic labeling;
- click chemistry;
- fatty acylation