Unit

Development of Chemical Probes for Biochemical Detection and Cellular Imaging of Myristoylated and Palmitoylated Proteins

  1. Rami N. Hannoush

Published Online: 1 FEB 2011

DOI: 10.1002/9780470559277.ch100143

Current Protocols in Chemical Biology

Current Protocols in Chemical Biology

How to Cite

Hannoush, R. N. 2011. Development of Chemical Probes for Biochemical Detection and Cellular Imaging of Myristoylated and Palmitoylated Proteins. Current Protocols in Chemical Biology. 3:15–26.

Author Information

  1. Genentech, South San Francisco, California

Publication History

  1. Published Online: 1 FEB 2011
  2. Published Print: FEB 2011

Abstract

Fatty acylation of proteins regulates their spatial localization and activity in living cells. Methods to monitor fatty acylation are invaluable for studying its role in regulating protein dynamics. The protocols in this unit describe a procedure that involves metabolic labeling with ω-alkynyl fatty acids for detecting and cellular imaging of fatty-acylated proteins, namely myristoylated and palmitoylated proteins. Curr. Protoc. Chem. Biol. 3:15-26 © 2011 by John Wiley & Sons, Inc.

Keywords:

  • myristoylation;
  • palmitoylation;
  • alkynyl fatty acids;
  • chemical probes;
  • metabolic labeling;
  • click chemistry;
  • fatty acylation