Unit

Derivatization of Free Natural Glycans for Incorporation onto Glycan Arrays: Derivatizing Glycans on the Microscale for Microarray and Other Applications

  1. Xuezheng Song,
  2. Jamie Heimburg-Molinaro,
  3. David F. Smith,
  4. Richard D. Cummings

Published Online: 1 APR 2011

DOI: 10.1002/9780470559277.ch100194

Current Protocols in Chemical Biology

Current Protocols in Chemical Biology

How to Cite

Song, X., Heimburg-Molinaro, J., Smith, D. F. and Cummings, R. D. 2011. Derivatization of Free Natural Glycans for Incorporation onto Glycan Arrays: Derivatizing Glycans on the Microscale for Microarray and Other Applications. Current Protocols in Chemical Biology. 3:53–63.

Author Information

  1. Emory University, Atlanta, Georgia

Publication History

  1. Published Online: 1 APR 2011
  2. Published Print: APR 2011

Abstract

Nature possesses an unlimited number and source of biologically relevant natural glycans, many of which are too complicated to synthesize in the laboratory. To capitalize on the naturally occurring plethora of glycans, a method is presented here to fluorescently tag isolated free glycans while maintaining the closed-ring structure. After purification of the labeled glycans, they can be printed on a glass surface to create a natural glycan microarray, suitable for interrogation with potential glycan-binding proteins. The derivatization of these natural glycans has vastly expanded the number of glycans available for functional studies. Curr. Protoc. Chem. Biol. 3:53-63 © 2011 by John Wiley & Sons, Inc.

Keywords:

  • fluorescence;
  • reductive amination;
  • glycan microarray;
  • conjugation