Copper-Catalyzed Azide–Alkyne Click Chemistry for Bioconjugation
Published Online: 1 DEC 2011
Copyright © 2011 by John Wiley and Sons, Inc.
Lab Protocol Title
Current Protocols in Chemical Biology
How to Cite
Presolski, S. I., Hong, V. P. and Finn, M. 2011. Copper-Catalyzed Azide–Alkyne Click Chemistry for Bioconjugation. Current Protocols in Chemical Biology. 3:153–162.
- Published Online: 1 DEC 2011
- Published Print: DEC 2011
The copper-catalyzed azide-alkyne cycloaddition reaction is widely used for the connection of molecular entities of all sizes. A protocol is provided here for the process with biomolecules. Ascorbate is used as reducing agent to maintain the required cuprous oxidation state. Since these convenient conditions produce reactive oxygen species, five equivalents of a copper-binding ligand are used with respect to metal. The ligand both accelerates the reaction and serves as a sacrificial reductant, protecting the biomolecules from oxidation. A procedure is also described for testing the efficiency of the reaction under desired conditions for purposes of optimization, before expensive biological reagents are used. Curr. Protoc. Chem. Biol. 3:153-162 © 2011 by John Wiley & Sons, Inc.
- click chemistry;
- nucleic acids;