Unit

On-Bead Two-Color (OBTC) Cell Screen for Direct Identification of Highly Selective Cell Surface Receptor Ligands

  1. D. Gomika Udugamasooriya1,2,
  2. Thomas Kodadek3,4

Published Online: 1 MAR 2012

DOI: 10.1002/9780470559277.ch110199

Current Protocols in Chemical Biology

Current Protocols in Chemical Biology

How to Cite

Udugamasooriya, D. G. and Kodadek, T. 2012. On-Bead Two-Color (OBTC) Cell Screen for Direct Identification of Highly Selective Cell Surface Receptor Ligands. Current Protocols in Chemical Biology. 4:35–48.

Author Information

  1. 1

    Advanced Imaging Research Center, University of Texas Southwestern Medical Center, Dallas, Texas

  2. 2

    Department of Biochemistry, University of Texas Southwestern Medical Center, Dallas, Texas

  3. 3

    Department of Chemistry, The Scripps Research Institute, Jupiter, Florida

  4. 4

    Department of Cancer Biology, The Scripps Research Institute, Jupiter, Florida

Publication History

  1. Published Online: 1 MAR 2012
  2. Published Print: MAR 2012

Abstract

Combinatorial library screens can identify a suitable ligand for a biological target of interest out of thousands or even millions of compounds, and can play a key role in the drug development process. While conventional high-throughput cell screens based on functional assays require expensive robotics, simple on-bead combinatorial assays for ligand binding to the target protein can be done far more cheaply. This unit describes one such assay, developed using combinatorial peptoid libraries for targeting integral membrane receptors or other cell surface-exposed molecules. In addition to the reduced cost, a unique advantage of this assay is the direct identification of the most selective ligands for a cell surface receptor that is expressed in its natural environment. Curr. Protoc. Chem. Biol. 4:35-48 © 2012 by John Wiley & Sons, Inc.

Keywords:

  • combinatorial;
  • cell screen;
  • receptor;
  • peptoid