Unit

Functional Assays on High-Content Protein Microarrays

  1. Lihao Meng

Published Online: 1 SEP 2012

DOI: 10.1002/9780470559277.ch110267

Current Protocols in Chemical Biology

Current Protocols in Chemical Biology

How to Cite

Meng, L. 2012. Functional Assays on High-Content Protein Microarrays. Current Protocols in Chemical Biology. 4:211–231.

Author Information

  1. Life Technologies, Carlsbad, California

Publication History

  1. Published Online: 1 SEP 2012

Abstract

High-content functional protein microarrays, such as ProtoArray Human Protein Array from Life Technologies, contain thousands of proteins or protein fragments of biological significance. Based on this platform, numerous assays have been developed to investigate protein-protein interactions, protein-DNA interactions, protein-phospholipid interactions, small-molecule targets, substrates of protein kinases, substrates of ubiquitin E3 ligases, and substrates of methyltransferase. This article provides a detailed illustration of the general procedure for setting up functional assays for high-throughput screening and also provides guidelines for data analysis. The protocols include the use of fluorescently labeled and radioactive probes for detection. Curr. Protoc. Chem. Biol. 4:211-231 © 2012 by John Wiley & Sons, Inc.

Keywords:

  • high content protein microarray;
  • functional assays;
  • protein-protein interaction;
  • small-molecule interaction;
  • kinase substrate profiling;
  • ubiquitin ligase substrate profiling;
  • protein methyltransferase substrate profiling