Unit

Advances in Lectin Microarray Technology: Optimized Protocols for Piezoelectric Print Conditions

  1. Kanoelani T. Pilobello1,
  2. Praveen Agrawal1,
  3. Richard Rouse2,
  4. Lara K. Mahal1

Published Online: 1 MAR 2013

DOI: 10.1002/9780470559277.ch120035

Current Protocols in Chemical Biology

Current Protocols in Chemical Biology

How to Cite

Pilobello, K. T., Agrawal, P., Rouse, R. and Mahal, L. K. 2013. Advances in Lectin Microarray Technology: Optimized Protocols for Piezoelectric Print Conditions. Current Protocols in Chemical Biology. 5:1–23.

Author Information

  1. 1

    Department of Chemistry, Biomedical Chemistry Institute, New York University, New York

  2. 2

    HTS Resources, San Diego, California

Publication History

  1. Published Online: 1 MAR 2013

Abstract

Lectin microarray technology has been used to profile the glycosylation of a multitude of biological and clinical samples, leading to new clinical biomarkers and advances in glycobiology. Lectin microarrays, which include >90 plant lectins, recombinant lectins, and selected antibodies, are used to profile N-linked, O-linked, and glycolipid glycans. The specificity and depth of glycan profiling depends upon the carbohydrate-binding proteins arrayed. The current set targets mammalian carbohydrates including fucose, high mannose, branched and complex N-linked, α- and β-galactose and GalNAc, α-2,3- and α-2,6-sialic acid, LacNAc, and Lewis X epitopes. Previous protocols have described the use of a contact microarray printer for lectin microarray production. Here, an updated protocol that uses a non-contact, piezoelectric printer, which leads to increased lectin activity on the array, is presented. Optimization of print and sample hybridization conditions and methods of analysis are discussed. Curr. Protoc. Chem. Biol. 5:1-23 © 2013 by John Wiley & Sons, Inc.

Keywords:

  • carbohydrate analysis;
  • glycomics;
  • lectin microarray;
  • Nano-Plotter;
  • piezoelectric