Standard Article

Plasmid DNA and Messenger RNA for Therapy

Pharmaceutical Biotechnology

  1. Steve Pascolo

Published Online: 15 MAR 2010

DOI: 10.1002/9780470571224.pse320

Pharmaceutical Sciences Encyclopedia

Pharmaceutical Sciences Encyclopedia

How to Cite

Pascolo, S. 2010. Plasmid DNA and Messenger RNA for Therapy. Pharmaceutical Sciences Encyclopedia. 31:1–42.

Author Information

  1. University of Tübingen, Institute for Cell Biology, Tübingen, Germany

Publication History

  1. Published Online: 15 MAR 2010


Recent studies performed in animal models and humans show that injection of pDNA or mRNA is safe and can elicit the expected results. Because pDNA and stabilized mRNA have the natural capacity to activate the immune system through the triggering of TLR, pDNA- and mRNA-based therapeutic utilizations are mostly tested in the field of vaccination. This article focuses on therapeutic applications of pDNA- and mRNA-based approaches.


  • plasmid DNA (pDNA);
  • messenger RNA (mRNA);
  • vaccination;
  • gene therapy