10. Assays with GPCRs

  1. Ge Wu

Published Online: 16 JUN 2010

DOI: 10.1002/9780470583128.ch10

Assay Development: Fundamentals and Practices

Assay Development: Fundamentals and Practices

How to Cite

Wu, G. (2010) Assays with GPCRs, in Assay Development: Fundamentals and Practices, John Wiley & Sons, Inc., Hoboken, NJ, USA. doi: 10.1002/9780470583128.ch10

Author Information

  1. La Canada Flintridge, California, USA

Publication History

  1. Published Online: 16 JUN 2010
  2. Published Print: 29 MAR 2010

ISBN Information

Print ISBN: 9780470191156

Online ISBN: 9780470583128

SEARCH

Keywords:

  • G Protein-Coupled Receptors, transmembrane proteins - assays with GPCRs;
  • GPCR assay development strategies and GPCR signaling processes;
  • G protein-coupled receptor assays - based on measurement of intracellular inositol phospholipids

Summary

This chapter contains sections titled:

  • Introduction to GPCRs and G Proteins

  • G Protein-Coupled Receptor Activation and Signal Transduction

  • Strategies of GPCR Assay Development

  • G Protein-Coupled Receptor Assays by Measuring the Extent of GTP Binding to Gα

  • G Protein-Coupled Receptor Assays Based on Measurement of cAMP

  • G Protein-Coupled Receptor Assays Based on Measurement of Intracellular Inositol Phospholipids

  • G Protein-Coupled Receptor Assays Based on Measurement of Intracellular Ca2+

  • G Protein-Coupled Receptor Assays Based on Measurement of MAPK Activity

  • G Protein-Coupled Receptor Assays with Reporter Gene

  • G Protein-Coupled Receptor Assays by Monitoring Events Leading to GPCR Internalization

  • Useful Websites

  • Bibliography