14. Case Study: Development of a Microfluidic-Based Kinase Assay Platform

  1. Ge Wu

Published Online: 16 JUN 2010

DOI: 10.1002/9780470583128.ch14

Assay Development: Fundamentals and Practices

Assay Development: Fundamentals and Practices

How to Cite

Wu, G. (2010) Case Study: Development of a Microfluidic-Based Kinase Assay Platform, in Assay Development: Fundamentals and Practices, John Wiley & Sons, Inc., Hoboken, NJ, USA. doi: 10.1002/9780470583128.ch14

Author Information

  1. La Canada Flintridge, California, USA

Publication History

  1. Published Online: 16 JUN 2010
  2. Published Print: 29 MAR 2010

ISBN Information

Print ISBN: 9780470191156

Online ISBN: 9780470583128

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Keywords:

  • microfluidic-based kinase assay platform development;
  • microfluidic kinase assay - ability in detecting inhibitors in a library;
  • alternative kinase assay method search

Summary

This chapter contains sections titled:

  • Background of Microfluidic Technology and Its Application in Bioassays

  • The Original Mobility Shift Kinase Assay Format

  • Realizing the Flaws in the Original Kinase Assay Format

  • Searching for Alternative Kinase Assay Methods

  • Development of the Off-Chip Kinase Assay Format

  • Current Stage of Microfluidic Technology in Bioassays

  • Appendix: Poster Presented at the 2002 Society for Biomolecular Screening Annual Meeting: Analysis of Mobility Shift Data Obtained from Labchip in Kinase Assay

  • Useful Websites

  • Bibliography