5. General Protein Binding Assay Formats

  1. Ge Wu

Published Online: 16 JUN 2010

DOI: 10.1002/9780470583128.ch5

Assay Development: Fundamentals and Practices

Assay Development: Fundamentals and Practices

How to Cite

Wu, G. (2010) General Protein Binding Assay Formats, in Assay Development: Fundamentals and Practices, John Wiley & Sons, Inc., Hoboken, NJ, USA. doi: 10.1002/9780470583128.ch5

Author Information

  1. La Canada Flintridge, California, USA

Publication History

  1. Published Online: 16 JUN 2010
  2. Published Print: 29 MAR 2010

ISBN Information

Print ISBN: 9780470191156

Online ISBN: 9780470583128

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Keywords:

  • general protein binding assay formats;
  • radioactive competitive binding assay - performed in SPA and FlashPlate formats;
  • Biacore technology application in binding studies

Summary

This chapter contains sections titled:

  • Equilibrium Dialysis

  • Competitive Binding Assays with Radioactive or Other Labeled Ligands

  • Application of SPA and FlashPlate in Binding Studies

  • Application of Fluorescence Polarization (FP) in Binding Studies

  • Application of FRET Assays in Binding Studies

  • Application of ELISA in Binding Studies

  • Surface Plasmon Resonance (SPR) Technology and Its Application in Binding Studies

  • Application of Label-Free Technologies in Binding Studies

  • Useful Websites

  • Bibliography