6. Functional Assays with Isolated Proteases

  1. Ge Wu

Published Online: 16 JUN 2010

DOI: 10.1002/9780470583128.ch6

Assay Development: Fundamentals and Practices

Assay Development: Fundamentals and Practices

How to Cite

Wu, G. (2010) Functional Assays with Isolated Proteases, in Assay Development: Fundamentals and Practices, John Wiley & Sons, Inc., Hoboken, NJ, USA. doi: 10.1002/9780470583128.ch6

Author Information

  1. La Canada Flintridge, California, USA

Publication History

  1. Published Online: 16 JUN 2010
  2. Published Print: 29 MAR 2010

ISBN Information

Print ISBN: 9780470191156

Online ISBN: 9780470583128

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Keywords:

  • functional assays with isolated proteases;
  • protease substrate profiling;
  • protease inhibitors - for assays, critical steps in addition to obtaining enzyme substrates

Summary

This chapter contains sections titled:

  • Introduction to Proteases and Their Substrates

  • Function of Proteases and Their Role in Drug Discovery

  • Protease Assays

  • Protease Substrate Profiling

  • Protease Inhibitors

  • Assay Development for Caspases with a Fluorogenic Substrate

  • Assay Development for Carboxypeptidase U (EC 3.4.17.20)

  • Useful Websites for Proteases

  • Bibliography