Determination of Aflatoxin M1 Using Liquid Chromatographic Method: Comparison Between Uncertainty Obtained from in House Validation Data and from Proficiency Test Data

  1. French College of Metrology
  1. C. Focardi,
  2. M. Nocentini,
  3. S. Ninci,
  4. P. Perrella and
  5. G. Biancalani

Published Online: 3 FEB 2010

DOI: 10.1002/9780470611371.ch2

Transverse Disciplines in Metrology

Transverse Disciplines in Metrology

How to Cite

French College of Metrology (2009) Determination of Aflatoxin M1 Using Liquid Chromatographic Method: Comparison Between Uncertainty Obtained from in House Validation Data and from Proficiency Test Data, in Transverse Disciplines in Metrology, ISTE, London, UK. doi: 10.1002/9780470611371.ch2

Author Information

  1. Istituto Zooprofilattico Sperimentale delle Regioni Lazio e Toscana - Sezione di Firenze, Via di Castelpulci 43, 50010 San Martino alla Palma, Florence, Italy. Tel +39055721308- Fax +390557311323

Publication History

  1. Published Online: 3 FEB 2010
  2. Published Print: 1 JAN 2009

ISBN Information

Print ISBN: 9781848210486

Online ISBN: 9780470611371

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Keywords:

  • proficiency testing;
  • linearity;
  • systematic error;
  • estimation;
  • precision

Summary

An HPLC method with fluorescence detection has been validated for the determination of aflatoxin M1 in milk samples. Certified Reference Materials and Spiked samples have been used for in house validation. These validation data have been applied for the uncertainty estimation with the bottom- up approach. Results obtained with this method have been compared with the expanded uncertainty determined from proficiency testing FAPAS.