2. Practical Considerations for Flow Cytometric Sorting of Stem Cells

  1. Awtar Krishan2,
  2. H. Krishnamurthy3,
  3. Satish Totey4
  1. Geoffrey W. Osborne

Published Online: 9 JUL 2012

DOI: 10.1002/9780470631119.ch2

Book Title

Applications of Flow Cytometry in Stem Cell Research and Tissue Regeneration

Applications of Flow Cytometry in Stem Cell Research and Tissue Regeneration

Additional Information

How to Cite

Osborne, G. W. (2010) Practical Considerations for Flow Cytometric Sorting of Stem Cells, in Applications of Flow Cytometry in Stem Cell Research and Tissue Regeneration (eds A. Krishan, H. Krishnamurthy and S. Totey), John Wiley & Sons, Inc., Hoboken, NJ, USA. doi: 10.1002/9780470631119.ch2

Editor Information

  1. 2

    University of Miami Miller School of Medicine, Miami, Florida

  2. 3

    Tata Institute of Fundamental Research, Bangalore, India

  3. 4

    Advance Neuroscience Allies Pvt. Ltd., Bangalore, India

Author Information

  1. Queensland Brain Institute and Australian Institute for Bioengineering and Nanotechnology, University of Queensland, St. Lucia, Australia

Publication History

  1. Published Online: 9 JUL 2012
  2. Published Print: 28 JUL 2010

ISBN Information

Print ISBN: 9780470543986

Online ISBN: 9780470631119

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CHAPTER TOOLS

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Keywords:

  • flow cytometric sorting;
  • stem cells;
  • cell suspension media;
  • cell sorter;
  • sterile cell sorting

Summary

This chapter contains sections titled:

  • Introduction

  • The Sorting Process

  • Preparation of the Instrument for Stem Cell Sorting

  • Cell Preparation

  • Instrument Setup, Sorting, and Data Collection

  • Analysis of Sorting Performance: Critical Aspects

  • Conclusions

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