Standard Article

van Slyke Method

(van Slyke Amino Nitrogen Determination, van Slyke Determination)

Published Online: 15 SEP 2010

DOI: 10.1002/9780470638859.conrr640

Comprehensive Organic Name Reactions and Reagents

Comprehensive Organic Name Reactions and Reagents

How to Cite

2010. van Slyke Method. Comprehensive Organic Name Reactions and Reagents. 640:2860–2863.

Publication History

  1. Published Online: 15 SEP 2010

Chemistry Terms

Choose one or more boxes to highlight terms.

Abstract

The chemical test to determine the amount of amino acids with a primary amino group in the hydrolysates of proteins and food-stuff, by the treatment of a sample in glycerol with sodium nitrite and acetic acid and a measurement of the volume of nitrogen gas that is generated, this method is generally referred to as the van Slyke determination. This method can be used to determine the amount of amino acids quantitatively, and the result is consistent with the determination using 2,4-dinitrofluorobenzene (DNFB, the Sanger method) and ninhydrin. However, there are certain amino acids in the protein, which cannot be determined. This method has wide application in amino acid analysis.

Keywords:

  • 2,4-dinitrofluorobenzene;
  • diazotinium salt;
  • ninhydrin;
  • tryptophan