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Transport Experiments in Membranes

Techniques

  1. Stefan Matile,
  2. Naomi Sakai,
  3. Andreas Hennig

Published Online: 15 MAR 2012

DOI: 10.1002/9780470661345.smc031

Supramolecular Chemistry: From Molecules to Nanomaterials

Supramolecular Chemistry: From Molecules to Nanomaterials

How to Cite

Matile, S., Sakai, N. and Hennig, A. 2012. Transport Experiments in Membranes. Supramolecular Chemistry: From Molecules to Nanomaterials. .

Author Information

  1. University of Geneva, Geneva, Switzerland

Publication History

  1. Published Online: 15 MAR 2012

Abstract

In this chapter, we describe methods to characterize the activity of synthetic transport systems in translocating molecules across otherwise impermeable membranes. The first two sections introduce methods with bulk membranes (U-tube experiments) and planar or “black” lipid membranes (BLMs), and close with a more comprehensive overview of methods involving liposomes, in particular, large unilamellar vesicles (LUVs). The fourth section focuses on the application of these methods to the elucidation of the functional characteristics of synthetic transport systems. This includes sensitivity toward membrane composition, membrane potential, pH, anions, cations, molecular recognition (sensing), molecular transformation (catalysis), or light (photosynthesis).

Keywords:

  • ion channels;
  • ion carriers;
  • pores;
  • sensors;
  • photosynthesis;
  • voltage gating;
  • ligand gating;
  • pH gating;
  • vesicles;
  • Hill analysis