Chapter 1. Changing Concepts in the Characterisation of Microbes and the Influence of Mass Spectrometry

  1. Haroun N. Shah and
  2. Saheer E. Gharbia
  1. Haroun N. Shah3,
  2. Caroline Chilton3,
  3. Lakshani Rajakaruna3,
  4. Tom Gaulton3,
  5. Gillian Hallas3,
  6. Hristo Atanassov1,
  7. Ghalia Khoder1,
  8. Paulina D. Rakowska2,
  9. Eleonora Cerasoli2 and
  10. Saheer E. Gharbia3

Published Online: 15 JUN 2010

DOI: 10.1002/9780470665497.ch1

Mass Spectrometry for Microbial Proteomics

Mass Spectrometry for Microbial Proteomics

How to Cite

Shah, H. N., Chilton, C., Rajakaruna, L., Gaulton, T., Hallas, G., Atanassov, H., Khoder, G., Rakowska, P. D., Cerasoli, E. and Gharbia, S. E. (2010) Changing Concepts in the Characterisation of Microbes and the Influence of Mass Spectrometry, in Mass Spectrometry for Microbial Proteomics (eds H. N. Shah and S. E. Gharbia), John Wiley & Sons, Ltd, Chichester, UK. doi: 10.1002/9780470665497.ch1

Editor Information

  1. Department for Bioanalysis and Horizon Technologies, Health Protection Agency Centre for Infections, 61 Colindale Avenue, London NW9 5EQ, UK

Author Information

  1. 1

    Pôle Biologie-Santé, Pavillon Médecine-Sud, Centre Hospitalier Universitaire–La Milétrie, 40, avenue du recteur Pineau, 86022 Poitiers Cedex, France

  2. 2

    Biophysics and Biodiagnostics Quality of Life Division, National Physical Laboratory, Hampton Road, Teddington TW11 0LW, UK

  3. 3

    Department for Bioanalysis and Horizon Technologies, Health Protection Agency Centre for Infections, 61 Colindale Avenue, London NW9 5EQ, UK

Publication History

  1. Published Online: 15 JUN 2010
  2. Published Print: 23 JUL 2010

ISBN Information

Print ISBN: 9780470681992

Online ISBN: 9780470665497

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Keywords:

  • microbe characterisation and mass spectrometry influence-changing concepts;
  • diversity and interrelationships between species-central to microbiology development;
  • chemical analysis of bacterial cell macromolecules;
  • sodium dodecyl sulfate polyacrylamide gel electrophoresis (SDS-PAGE);
  • Bergery's Manual of Systematic Bacteriology-changing format, reflecting this pattern;
  • microorganism characterisation by MALDI-TOF-MS;
  • TOF-MS experiments-development of databases for pattern retrieval and profile matching;
  • proteomes of diverse strains within species-comparative analysis;
  • nanoparticles, alternative approach in analysis and low abundance and low molecular weight protein detection;
  • new technologies-major impact elucidating complex mechanisms in host–bacterial interaction

Summary

This chapter contains sections titled:

  • Background and Early Attempts to Use Mass Spectrometry on Microbes

  • Characterisation of Microorganisms by MALDI-TOF-MS; from Initial Ideas to the Development of the First Comprehensive Database

  • Characterisation of Microorganisms from Their Intracellular/Membrane Bound Protein Profiles Using Affinity Capture with Particular Reference to Surface-Enhanced Laser Desorption/Ionisation (SELDI)-TOF-MS

  • Comparative Analysis of Proteomes of Diverse Strains within a Species; Use of 2D Fluorescence Difference Gel Electrophoresis (DIGE)

  • Nanoparticles as an Alternative Approach in the Analysis and Detection of Low Abundance and Low Molecular Weight Proteins Using MALDI-TOF-MS

  • References