Chapter 15. The Proteome of Francisella Tularensis; Methodology And Mass Spectral Analysis for Studying One of the Most Dangerous Human Pathogens

  1. Haroun N. Shah and
  2. Saheer E. Gharbia
  1. Jiri Stulik,
  2. Juraj Lenco,
  3. Jiri Dresler,
  4. Jana Klimentova,
  5. Lenka Hernychova,
  6. Lucie Balonova,
  7. Alena Fucikova and
  8. Marek Link

Published Online: 15 JUN 2010

DOI: 10.1002/9780470665497.ch15

Mass Spectrometry for Microbial Proteomics

Mass Spectrometry for Microbial Proteomics

How to Cite

Stulik, J., Lenco, J., Dresler, J., Klimentova, J., Hernychova, L., Balonova, L., Fucikova, A. and Link, M. (2010) The Proteome of Francisella Tularensis; Methodology And Mass Spectral Analysis for Studying One of the Most Dangerous Human Pathogens, in Mass Spectrometry for Microbial Proteomics (eds H. N. Shah and S. E. Gharbia), John Wiley & Sons, Ltd, Chichester, UK. doi: 10.1002/9780470665497.ch15

Editor Information

  1. Department for Bioanalysis and Horizon Technologies, Health Protection Agency Centre for Infections, 61 Colindale Avenue, London NW9 5EQ, UK

Author Information

  1. Institute of Molecular Pathology, Faculty of Military Health Science UO, Trebesska 1575, 500 01 Hradec Kralove, Czech Republic

Publication History

  1. Published Online: 15 JUN 2010
  2. Published Print: 23 JUL 2010

ISBN Information

Print ISBN: 9780470681992

Online ISBN: 9780470665497

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Keywords:

  • proteome of Francisella tularensis-methodology and mass spectral analysis, most dangerous human pathogens;
  • molecular pathogenesis of Francisella tularensis infection;
  • Francisella tularensis LVS proteome alterations-induced by temperature differences and stationary phase of growth;
  • F. tularensis-membrane protein complex analysis;
  • analysis of oxidative phosphorylation membrane protein complexes of F. tularensis - by 2D BN/SDS-PAGE;
  • identification of F. tularensis transcription factors involved in its virulence;
  • new virulence factors and new genetic tools-new plasmids and transposons;
  • post-translational modifications (PTMs) of proteins-role in assembly, degradation, structure and function of expressed genes

Summary

This chapter contains sections titled:

  • Introduction to Molecular Pathogenesis of Francisella tularensis Infection

  • Francisella tularensis LVS Proteome Alterations Induced by Different Temperatures and Stationary Phase of Growth

  • Analysis of Membrane Protein Complexes of F. tularensis

  • Analysis of F. tularensis Glycoproteins and Phosphoproteins

  • Identification of F. tularensis Transcription Factors Potentially Involved in Its Virulence

  • Acknowledgements

  • References