Chapter 20. Transfer of a Traditional Serotyping System (Kauffmann–White) onto a MALDI-TOF-MS Platform for the Rapid Typing of Salmonella Isolates

  1. Haroun N. Shah and
  2. Saheer E. Gharbia
  1. Chloe Bishop,
  2. Catherine Arnold and
  3. Saheer E. Gharbia

Published Online: 15 JUN 2010

DOI: 10.1002/9780470665497.ch20

Mass Spectrometry for Microbial Proteomics

Mass Spectrometry for Microbial Proteomics

How to Cite

Bishop, C., Arnold, C. and Gharbia, S. E. (2010) Transfer of a Traditional Serotyping System (Kauffmann–White) onto a MALDI-TOF-MS Platform for the Rapid Typing of Salmonella Isolates, in Mass Spectrometry for Microbial Proteomics (eds H. N. Shah and S. E. Gharbia), John Wiley & Sons, Ltd, Chichester, UK. doi: 10.1002/9780470665497.ch20

Editor Information

  1. Department for Bioanalysis and Horizon Technologies, Health Protection Agency Centre for Infections, 61 Colindale Avenue, London NW9 5EQ, UK

Author Information

  1. Department for Bioanalysis and Horizon Technologies, Health Protection Agency Centre for Infections, 61 Colindale Avenue, London NW9 5EQ, UK

Publication History

  1. Published Online: 15 JUN 2010
  2. Published Print: 23 JUL 2010

ISBN Information

Print ISBN: 9780470681992

Online ISBN: 9780470665497

SEARCH

Keywords:

  • traditional serotyping system transfer (Kauffmann–White)-onto MALDI-TOF-MS platform for rapid typing of Salmonella isolates;
  • DNA sequence-based approaches-giving flexibility to target selective gene marking for organisms;
  • Salmonella, a food- and water-borne pathogen-easily disseminated in populations;
  • Salmonella strain pathogenicity-depending on virulence factor combination;
  • Salmonellosis, and syndromes-gastroenteritis, enteric fevers, septicaemia, focal infections and asymptomatic carrier state;
  • antigenic analysis-Traditional Kauffmann–White scheme and its future;
  • somatic antigens and Structural Variation of the O-Antigen;
  • sequence-based methods in determining serotypes;
  • DNA sequencing using MALDI-TOF-MS instruments-a different approach;
  • O-groups identification-gene sequences of rfb cluster, for sero-specific motifs

Summary

This chapter contains sections titled:

  • Introduction

  • Salmonella, the Pathogen

  • Complex Genetic Structure and the Need to Subtype this Genus

  • Antigenic Analysis – the Traditional Kauffmann–White Scheme and Its Future

  • Sequence-Based Methods to Determine Serotypes

  • Transferring to a MALDI Platform for Rapid Analysis

  • Conclusions and Summary

  • References