Chapter 4. Tandem Mass Spectrometry-Based Proteomics, Protein Characterisation and Biomarker Discovery in Microorganisms

  1. Haroun N. Shah and
  2. Saheer E. Gharbia
  1. Min Fang

Published Online: 15 JUN 2010

DOI: 10.1002/9780470665497.ch4

Mass Spectrometry for Microbial Proteomics

Mass Spectrometry for Microbial Proteomics

How to Cite

Fang, M. (2010) Tandem Mass Spectrometry-Based Proteomics, Protein Characterisation and Biomarker Discovery in Microorganisms, in Mass Spectrometry for Microbial Proteomics (eds H. N. Shah and S. E. Gharbia), John Wiley & Sons, Ltd, Chichester, UK. doi: 10.1002/9780470665497.ch4

Editor Information

  1. Department for Bioanalysis and Horizon Technologies, Health Protection Agency Centre for Infections, 61 Colindale Avenue, London NW9 5EQ, UK

Author Information

  1. Department for Bioanalysis and Horizon Technologies, Health Protection Agency Centre for Infections, 61 Colindale Avenue, London NW9 5EQ, UK

Publication History

  1. Published Online: 15 JUN 2010
  2. Published Print: 23 JUL 2010

ISBN Information

Print ISBN: 9780470681992

Online ISBN: 9780470665497

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Keywords:

  • tandem mass spectrometry-based proteomics, protein characterisation and biomarker discovery in microorganisms;
  • mass spectrometry-MALDI versus ESI;
  • proteomic strategies for protein identification;
  • multidimensional protein identification;
  • mass spectrometry-based targeted protein quantification and biomarker discovery;
  • stable isotope-labelled peptides-as internal standards coupled with selected reaction monitoring (SRM);
  • proteomics, mass spectrometry (MS)-based proteomics-an indispensable technology;
  • ECD and ETD technique development-improving dissociation efficiency for intact protein molecules;
  • Tandem MS-based proteomics, combined with sample preparation and separation techniques

Summary

This chapter contains sections titled:

  • Introduction

  • Mass Spectrometry

  • Proteomic Strategies for Protein Identification

  • Multidimensional Protein Identification

  • Mass Spectrometry-Based Targeted Protein Quantification and Biomarker Discovery

  • Conclusions

  • References