Chapter 6. Conventional Approaches for Sample Preparation for Liquid Chromatography and Two-Dimensional Gel Electrophoresis

  1. Haroun N. Shah and
  2. Saheer E. Gharbia
  1. Vesela Encheva1 and
  2. Robert Parker2

Published Online: 15 JUN 2010

DOI: 10.1002/9780470665497.ch6

Mass Spectrometry for Microbial Proteomics

Mass Spectrometry for Microbial Proteomics

How to Cite

Encheva, V. and Parker, R. (2010) Conventional Approaches for Sample Preparation for Liquid Chromatography and Two-Dimensional Gel Electrophoresis, in Mass Spectrometry for Microbial Proteomics (eds H. N. Shah and S. E. Gharbia), John Wiley & Sons, Ltd, Chichester, UK. doi: 10.1002/9780470665497.ch6

Editor Information

  1. Department for Bioanalysis and Horizon Technologies, Health Protection Agency Centre for Infections, 61 Colindale Avenue, London NW9 5EQ, UK

Author Information

  1. 1

    Research and Technology Department, Laboratory of the Government Chemist, Queens Road, Teddington, Middlesex, TW11 OLY, UK

  2. 2

    University of British Columbia, Center for High-Throughput Biology, Vancouver, Canada, V6T, 1Z4

Publication History

  1. Published Online: 15 JUN 2010
  2. Published Print: 23 JUL 2010

ISBN Information

Print ISBN: 9780470681992

Online ISBN: 9780470665497

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Keywords:

  • protein samples-preparation techniques;
  • sample preparation for liquid chromatography and two-dimensional gel electrophoresis-conventional approaches;
  • 2D GE-mark on elucidating mechanisms of bacterial pathogenicity, antibiotics resistance, physiology and vaccine development;
  • cell lysis methods and mechanical lysis;
  • sonication, applying sound energy-to agitate particles in sample;
  • French pressure cell, most effective systems for cell disruption of bacterial cells;
  • chemical and osmotic lysis;
  • sample preparation for 2D GE;
  • fractionation strategies and surface associated proteins;
  • microscale clean up prior to LC-MS

Summary

This chapter contains sections titled:

  • Introduction

  • Cell Lysis Methods

  • Sample Preparation for 2D GE

  • Fractionation Strategies

  • Sample Preparation for Liquid Chromatography coupled with Mass Spectrometry (LC-MS)

  • Conclusion

  • References