Chapter 2. Microarray Platforms and Aspects of Experimental Variation
- Andreas Scherer Founder/CEO of Spheromics
Published Online: 2 NOV 2009
DOI: 10.1002/9780470685983.ch2
Copyright © 2009 John Wiley & Sons, Ltd
Book Title
Batch Effects and Noise in Microarray Experiments: Sources and Solutions
Additional Information
How to Cite
Coller, J. A. (2009) Microarray Platforms and Aspects of Experimental Variation, in Batch Effects and Noise in Microarray Experiments: Sources and Solutions (ed A. Scherer), John Wiley & Sons, Ltd, Chichester, UK. doi: 10.1002/9780470685983.ch2
Editor Information
Spheromics, Kontiolahti, Finland
Publication History
- Published Online: 2 NOV 2009
- Published Print: 30 OCT 2009
Book Series:
ISBN Information
Print ISBN: 9780470741382
Online ISBN: 9780470685983
- Summary
- Chapter
Keywords:
- microarray technology;
- platforms;
- production;
- sample processing;
- sources of variability;
- bias
Summary
In the early 1990s, a number of technologies were developed that made it possible to investigate gene expression in a high-throughput fashion (Adams et al. 1991; Liang and Pardee 1992; Pease et al. 1994; Schena et al. 1995; Velculescu et al. 1995). Of these technologies, the DNA microarray has become the standard tool for investigating genome-wide gene expression levels and has revolutionized the way scientists investigate the genome. This chapter will give a brief overview of current microarray technologies that are being used today. The chapter will also go through a typical experimental procedure discussing various sources of experimental variability and bias that may affect the data generated and experimental results.