Chapter 2. Microarray Platforms and Aspects of Experimental Variation

  1. Andreas Scherer Founder/CEO of Spheromics
  1. John A Coller Jr

Published Online: 2 NOV 2009

DOI: 10.1002/9780470685983.ch2

Batch Effects and Noise in Microarray Experiments: Sources and Solutions

Batch Effects and Noise in Microarray Experiments: Sources and Solutions

How to Cite

Coller, J. A. (2009) Microarray Platforms and Aspects of Experimental Variation, in Batch Effects and Noise in Microarray Experiments: Sources and Solutions (ed A. Scherer), John Wiley & Sons, Ltd, Chichester, UK. doi: 10.1002/9780470685983.ch2

Editor Information

  1. Spheromics, Kontiolahti, Finland

Author Information

  1. Stanford Functional Genomics Facility, Stanford University, Stanford, CA, USA

Publication History

  1. Published Online: 2 NOV 2009
  2. Published Print: 30 OCT 2009

ISBN Information

Print ISBN: 9780470741382

Online ISBN: 9780470685983

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Keywords:

  • microarray technology;
  • platforms;
  • production;
  • sample processing;
  • sources of variability;
  • bias

Summary

In the early 1990s, a number of technologies were developed that made it possible to investigate gene expression in a high-throughput fashion (Adams et al. 1991; Liang and Pardee 1992; Pease et al. 1994; Schena et al. 1995; Velculescu et al. 1995). Of these technologies, the DNA microarray has become the standard tool for investigating genome-wide gene expression levels and has revolutionized the way scientists investigate the genome. This chapter will give a brief overview of current microarray technologies that are being used today. The chapter will also go through a typical experimental procedure discussing various sources of experimental variability and bias that may affect the data generated and experimental results.