Chapter 10. Reversed-Phase Chromatography

  1. Veronika R. Meyer

Published Online: 9 APR 2010

DOI: 10.1002/9780470688427.ch10

Practical High-Performance Liquid Chromatography, Fifth Edition

Practical High-Performance Liquid Chromatography, Fifth Edition

How to Cite

Meyer, V. R. (2010) Reversed-Phase Chromatography, in Practical High-Performance Liquid Chromatography, Fifth Edition, John Wiley & Sons, Ltd, Chichester, UK. doi: 10.1002/9780470688427.ch10

Author Information

  1. Swiss Federal Laboratories for Materials Testing and Research (EMPA), St. Gallen, Switzerland

Publication History

  1. Published Online: 9 APR 2010
  2. Published Print: 9 APR 2010

ISBN Information

Print ISBN: 9780470682180

Online ISBN: 9780470688427

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Keywords:

  • reversed-phase chromatography;
  • reversed-phase chromatography - term describing the state in which the stationary phase is less polar;
  • chemically bonded octadecylsilane (ODS) - an n-alkane with 18 carbon atoms;
  • mobile phase - consisting of mixtures of water or aqueous buffer solutions;
  • nonaqueous eluents - needed for reversed-phase chromatography;
  • disadvantage of producing a relatively highly viscous mixture in methanol - with water (see above), giving rise to much higher pressures than other mobile phases;
  • eluent - important in adsorption chromatography;
  • reversed-phase stationary phases - hydrophobic

Summary

This chapter contains sections titled:

  • Principle

  • Mobile phases in reversed-phase chromatography

  • Solvent selectivity and strength

  • Stationary phases

  • Method development in reversed-phase chromatography

  • Applications

  • Hydrophobic interaction chromatography