Chapter 8. Tandem MS or MS/MS Analysis

  1. Ingvar Eidhammer1,
  2. Kristian Flikka1,
  3. Lennart Martens2 and
  4. Svein-Ole Mikalsen3

Published Online: 10 DEC 2007

DOI: 10.1002/9780470724309.ch8

Computational Methods for Mass Spectrometry Proteomics

Computational Methods for Mass Spectrometry Proteomics

How to Cite

Eidhammer, I., Flikka, K., Martens, L. and Mikalsen, S.-O. (2007) Tandem MS or MS/MS Analysis, in Computational Methods for Mass Spectrometry Proteomics, John Wiley & Sons, Ltd, Chichester, UK. doi: 10.1002/9780470724309.ch8

Author Information

  1. 1

    University of Bergen, Norway

  2. 2

    European Bioinformatics Institute, Hinxton, Cambridge, UK

  3. 3

    The Norwegian Radium Hospital, Oslo, Norway

Publication History

  1. Published Online: 10 DEC 2007
  2. Published Print: 30 NOV 2007

ISBN Information

Print ISBN: 9780470512975

Online ISBN: 9780470724309

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Keywords:

  • fragmentation spectrum or product ion spectrum;
  • collisionally activated dissociation (CAD);
  • quadrupole ion trap (QIT) and linear ion trap (LIT);
  • multiple reaction monitoring (MRM);
  • multiple charged precursors

Summary

This chapter contains sections titled:

  • Peptide fragments

  • Fragmentation techniques

  • MS/MS spectrometers

  • Different types of analyzers

  • Overview of the process for MS/MS analysis

  • Fragment ion masses and residue masses

  • Deisotoping and charge state deconvolution

  • Precursor treatment

  • MS3 spectra

  • Exercises

  • Bibliographic notes