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Application of Quantitative Proteomic Approaches to Toxicology

Systems Toxicology

Proteomic Technology

  1. T.S. Keshava Prasad1,
  2. Raghothama Chaerkady2

Published Online: 15 SEP 2011

DOI: 10.1002/9780470744307.gat214

General, Applied and Systems Toxicology

General, Applied and Systems Toxicology

How to Cite

Prasad, T. K. and Chaerkady, R. 2011. Application of Quantitative Proteomic Approaches to Toxicology. General, Applied and Systems Toxicology. .

Author Information

  1. 1

    Institute of Bioinformatics, Bangalore, India

  2. 2

    Johns Hopkins University, McKusick-Nathans Institute of Genetic Medicine and Departments of Biological Chemistry, Pathology and Oncology, Baltimore, MD, USA

Publication History

  1. Published Online: 15 SEP 2011

Abstract

Epidemiological and genetic studies have significantly contributed to our understanding of hazardous effects of various environmental pollutants on biological systems at a molecular level. However, only limited reports exist those describe proteomic alterations associated with toxicity of such pollutants. Proteomic signatures can be employed as biomarkers for exposure as well as toxicity. Improved protein/peptide labeling technologies have led to the development of a variety of methodologies for quantitative proteomics that can be used to obtain differential protein profiles. This review highlights various quantitative proteomic technologies, which can be applied to unravel proteomic changes in response to toxicants.

Keywords:

  • biomarkers;
  • clinical proteomics;
  • iTRAQ;
  • Arsenic;
  • mass spectrometry;
  • signaling pathways