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Use of Proteomic and Metabolomic Techniques in Ecotoxicological Research

Systems Toxicology

Proteomic Technology

  1. Maria S. Sepúlveda1,
  2. Kimberly J. Ralston-Hooper2,
  3. Brian C. Sanchez3,
  4. Amber Hopf-Jannasch4,
  5. Stephanie D. Baker5,
  6. Naomi Diaz6,
  7. Jiri Adamec4

Published Online: 15 SEP 2011

DOI: 10.1002/9780470744307.gat215

General, Applied and Systems Toxicology

General, Applied and Systems Toxicology

How to Cite

Sepúlveda, M. S., Ralston-Hooper, K. J., Sanchez, B. C., Hopf-Jannasch, A., Baker, S. D., Diaz, N. and Adamec, J. 2011. Use of Proteomic and Metabolomic Techniques in Ecotoxicological Research. General, Applied and Systems Toxicology. .

Author Information

  1. 1

    Purdue University, Department of Natural Resources, West Lafayette, IN, USA

  2. 2

    United States Environmental Protection Agency, Ecosystem Research Division, Athens, GA, USA

  3. 3

    Colorado Ecological Services Field Office, US Fish and Wildlife Service, Lakewood, CO, USA

  4. 4

    Purdue University, Bindley Biological Sciences Center, West Lafayette, IN, USA

  5. 5

    GEI Consultants Inc., Denver, CO, USA

  6. 6

    Purdue University, Department of Chemistry, West Lafayette, IN, USA

Publication History

  1. Published Online: 15 SEP 2011

Abstract

Over the last decade, the environmental sciences have witnessed an incredible movement towards the utilization of high through-put molecular tools that are capable of detecting simultaneous changes of hundreds and even thousands of molecules and molecular components after exposure of organisms to different environmental stressors. These techniques have received lots of attention because they not only offer the potential to unravel novel mechanisms of physiological and toxic action, but they also are amenable for the discovery of biomarkers of exposure and effects. In this chapter we will review the state of knowledge of two of these holistic tools in ecotoxicological research: Proteomics and metabolomics. We will follow this review with a presentation of three of our own case studies utilizing proteomic and metabolomic tools: (i) Analysis of proteomic responses in fish exposed to different types of contaminants using GeneGo™; (ii) Comparison of proteomic and metabolomic responses in aquatic invertebrates exposed to herbicides; and (ii) Use of metabolomics to characterize egg quality in fish eating birds exposed to persistent organic pollutants. We will end with some ideas for future studies and research needs.

Keywords:

  • proteomics;
  • metabolomics;
  • ecotoxicology;
  • fish;
  • invertebrates;
  • birds;
  • aquatic;
  • wildlife;
  • biomarkers;
  • contaminants;
  • toxicity;
  • pollution