15. Mapping Protein Expression on Pulmonary Vascular Endothelium

  1. Norbert F. Voelkel2 and
  2. Sharon Rounds Chief3,4
  1. Kerri A. Massey and
  2. Jan E. Schnitzer

Published Online: 11 SEP 2009

DOI: 10.1002/9780470747490.ch15

The Pulmonary Endothelium: Function in health and disease

The Pulmonary Endothelium: Function in health and disease

How to Cite

Massey, K. A. and Schnitzer, J. E. (2009) Mapping Protein Expression on Pulmonary Vascular Endothelium, in The Pulmonary Endothelium: Function in health and disease (eds N. F. Voelkel and S. Rounds), John Wiley & Sons, Ltd, Chichester, UK. doi: 10.1002/9780470747490.ch15

Editor Information

  1. 2

    The E. Raymond Fenton Professor of Pulmonary Research, Director, Victoria Johnson Center for Pulmonary Obstructive Disease Research, Pulmonary and Critical Care Medicine Division, Virginia Commonwealth University, Richmond, VA 23298, USA

  2. 3

    Alpert Medical School of Brown University, Medical Service, Providence VA, USA

  3. 4

    Medical Center, Providence, RI 02908, USA

Author Information

  1. Protogenomics Research Institute for Systems Medicine, Sidney Kimmel Cancer Center, San Diego, CA 92121, USA

Publication History

  1. Published Online: 11 SEP 2009
  2. Published Print: 16 OCT 2009

ISBN Information

Print ISBN: 9780470723616

Online ISBN: 9780470747490

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Keywords:

  • mapping protein expression on pulmonary vascular endothelium;
  • segmental differences of EC;
  • identifying molecular components of ECs in vivo;
  • phage libraries;
  • laser capture microdissection;
  • chemical labeling of surface proteins in vivo;
  • caveolae as transport vesicles;
  • endothelial nitric oxide synthase (eNOS)

Summary

This chapter contains sections titled:

  • Introduction

  • Segmental Differences of EC

  • Difficulties in Studying ECs

  • ECS in Culture

  • Defining EC Expression in vivo

  • Phage Libraries

  • Large-Scale Approaches

  • Purifying ECs

  • Laser Capture Microdissection

  • Chemical Labeling of Surface Proteins in vivo

  • Colloidal Silica Nanoparticles

  • Towards Comprehensive Protein Identification

  • Caveolae as Transport Vesicles

  • Caveolae in Mechanotransduction

  • Identification of Lung-Specific Proteins

  • Therapeutic Implications

  • Conclusions and Perspectives

  • References