Chapter 15. In Vivo and In Vitro Models for Nanotoxicology Testing

  1. Saura C. Sahu4 and
  2. Daniel A. Casciano5
  1. Rosalba Gornati1,
  2. Elena Papis1,
  3. Mario Di Gioacchino2,3,
  4. Enrico Sabbioni1,
  5. Isabella Dalle-Donne1,
  6. Aldo Milzani1 and
  7. Giovanni Bernardini1

Published Online: 18 AUG 2009

DOI: 10.1002/9780470747803.ch15



How to Cite

Gornati, R., Papis, E., Di Gioacchino, M., Sabbioni, E., Dalle-Donne, I., Milzani, A. and Bernardini, G. (2009) In Vivo and In Vitro Models for Nanotoxicology Testing, in Nanotoxicity (eds S. C. Sahu and D. A. Casciano), John Wiley & Sons, Ltd, Chichester, UK. doi: 10.1002/9780470747803.ch15

Editor Information

  1. 4

    Division of Toxicology, Center for Food Safety and Applied Nutrition, Food and Drug Administration, 8301 Muirkirk Road, Laurel, MD 20708, USA

  2. 5

    Department for Applied Science, University of Arkansas at Little Rock, Little Rock, AR, USA

Author Information

  1. 1

    Dipartimento di Biotecnologie e Scienze Molecolari, Università dell'Insubria, Dipartimento di Biologia, Università di Milano, CiSE, Università di Chieti, Italy

  2. 2

    G. d'Annunzio University Foundation, Ageing Research Center (CeSI), Chieti, Italy

  3. 3

    Dipartimento di Biotecnologie e Scienze Molecolari, Università dell'Insubria, Varese, Italy

Publication History

  1. Published Online: 18 AUG 2009
  2. Published Print: 21 AUG 2009

ISBN Information

Print ISBN: 9780470741375

Online ISBN: 9780470747803



  • in vivo and in vitro models for nanotoxicology testing;
  • nanotechnology and possible drawbacks;
  • nanofilms encapsulating macromolecules and polypeptide nanofilms;
  • antibacterial properties of silver NPs and NP shape;
  • scanning near-field optical microscope (SNOM);
  • electron energy loss spectroscopy (EELS);
  • atomic force microscope for nanomaterials;
  • metal NPs and inductively coupled plasma mass spectrometry (ICP-MS);
  • ICP-MS spectrometers - solid and liquid samples;
  • 3Rs - ethical basis for nanotoxicology


This chapter contains sections titled:

  • Nanotechnology: Very Promising, But a Little Scary

  • Is Shape Important?

  • Dynamic Light Scattering and Nanoparticle Tracking Analysis

  • Zeta Potential

  • Dissolution

  • In Vitro and In Vivo Models are Both Necessary for Nanotoxicology Research

  • 3Rs: An Ethical Basis for Nanotoxicology

  • Particle Characteristics Affecting Nanoparticle Toxicity: In Vivo and In Vivo Studies

  • NPs and Gene Expression

  • Nanoparticle Cytotoxicity and Oxidative Stress

  • Conclusions

  • Acknowledgements

  • References