Chapter 10. Reduction of Functional Groups

  1. John Whittall8 and
  2. Peter Sutton9
  1. Andrew S. Lamm1,
  2. Arshdeep Khare1,
  3. John P. N. Rosazza1,
  4. Andreas Taglieber2,
  5. Frank Schulz2,
  6. Frank Hollmann2,
  7. Monika Rusek2,
  8. Manfred T. Reetz2,
  9. Thierry Gefflaut3,
  10. Emmanuelle Sagot3,
  11. Jean Bolte3,
  12. Hisaaki Mihara4,
  13. Nobuyoshi Esaki5,
  14. Philip Conway6,
  15. Francesca Paradisi7 and
  16. Paul Engel6

Published Online: 15 DEC 2009

DOI: 10.1002/9780470748589.ch10

Practical Methods for Biocatalysis and Biotransformations

Practical Methods for Biocatalysis and Biotransformations

How to Cite

Lamm, A. S., Khare, A., Rosazza, J. P. N., Taglieber, A., Schulz, F., Hollmann, F., Rusek, M., Reetz, M. T., Gefflaut, T., Sagot, E., Bolte, J., Mihara, H., Esaki, N., Conway, P., Paradisi, F. and Engel, P. (2009) Reduction of Functional Groups, in Practical Methods for Biocatalysis and Biotransformations (eds J. Whittall and P. Sutton), John Wiley & Sons, Ltd, Chichester, UK. doi: 10.1002/9780470748589.ch10

Editor Information

  1. 8

    Manchester Interdisciplinary Biocentre, University of Manchester, United Kingdom

  2. 9

    GlaxoSmithKline, Synthetic Chemistry, Gunnels Wood Road, Stevenage, Hertfordshire SG1 2NY, UK

Author Information

  1. 1

    Center for Biocatalysis and Bioprocessing, 2501 Crosspark Road, Suite C100 MTF, University of Iowa, Iowa City, Iowa, IA 52242-5000, USA

  2. 2

    Max-Planck-Institut für Kohlenforschung, Kaiser-Wilhelm-Platz 1, 45470 Mülheim/Ruhr, Germany

  3. 3

    Department of Chemistry, Université Blaise Pascal, Clermont-Ferrand, France

  4. 4

    Department of Biotechnology, Institute of Science and Engineering, College of Life Sciences, Ritsumeikan University, Kusatsu, Shiga 525-8577, Japan

  5. 5

    Institute for Chemical Research, Kyoto University, Uji, Kyoto 611-0011, Japan

  6. 6

    School of Biomolecular and Biomedical Science, University College Dublin, Belfield, Dublin 4, Ireland

  7. 7

    School of Chemistry and Chemical Biology, University College Dublin, Belfield, Dublin 4, Ireland

Publication History

  1. Published Online: 15 DEC 2009
  2. Published Print: 18 DEC 2009

ISBN Information

Print ISBN: 9780470519271

Online ISBN: 9780470748589



  • reduction of functional groups;
  • biocatalytic reduction of carboxylic acids to respective aldehydes or alcohols - new biocatalytic process;
  • light-driven stereoselective biocatalytic oxidations and reductions;
  • sacrificial electron donor (ethylenediaminetetraacetic acid (EDTA));
  • expression and purification of PAMO-P3;
  • light-driven PAMO-P3-catalyzed Baeyer–Villiger oxidations;
  • light-driven YqjM-catalyzed reduction of ketoisophorone;
  • aminotransferases (ATs) catalysing stereoselective transfer of amino group from donor substrate;
  • cysteine sulfinic acid (CSA) as amino donor substrate, CSA;
  • enzymatic synthesis of L-4-fluorophenylalanine


This chapter contains sections titled:

  • Reduction of Carboxylic Acids by Carboxylic Acid Reductase Heterologously Expressed in Escherichia coli

  • Light-driven Stereoselective Biocatalytic Oxidations and Reductions

  • Unnatural Amino Acids by Enzymatic Transamination: Synthesis of Glutamic Acid Analogues with Aspartate Aminotransferase

  • Synthesis of L-Pipecolic Acid with Δ1-Piperidine-2-carboxylate Reductase from Pseudomonas putida

  • Synthesis of Substituted Derivatives of L-Phenylalanine and of other Non-natural L-Amino Acids Using Engineered Mutants of Phenylalanine Dehydrogenase