Chapter 2. Biocatalyst Identification and Scale-Up: Molecular Biology for Chemists

  1. John Whittall2 and
  2. Peter Sutton3
  1. Kathleen H. McClean

Published Online: 15 DEC 2009

DOI: 10.1002/9780470748589.ch2

Practical Methods for Biocatalysis and Biotransformations

Practical Methods for Biocatalysis and Biotransformations

How to Cite

McClean, K. H. (2009) Biocatalyst Identification and Scale-Up: Molecular Biology for Chemists, in Practical Methods for Biocatalysis and Biotransformations (eds J. Whittall and P. Sutton), John Wiley & Sons, Ltd, Chichester, UK. doi: 10.1002/9780470748589.ch2

Editor Information

  1. 2

    Manchester Interdisciplinary Biocentre, University of Manchester, United Kingdom

  2. 3

    GlaxoSmithKline, Synthetic Chemistry, Gunnels Wood Road, Stevenage, Hertfordshire SG1 2NY, UK

Author Information

  1. C-Tech Innovation Ltd, Capenhurst Technology Park, Capenhurst, Chester, CH1 6EH, UK

Publication History

  1. Published Online: 15 DEC 2009
  2. Published Print: 18 DEC 2009

ISBN Information

Print ISBN: 9780470519271

Online ISBN: 9780470748589

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Keywords:

  • biocatalyst identification and scale-up - molecular biology for chemists;
  • biotechnology, modern discipline with ancient origins;
  • potential biocatalysts for chemical synthesis;
  • metagenomics - sampling DNA directly from environment;
  • enzyme tools in molecular biology;
  • molecular cloning and rDNA;
  • schematic representation of typical simple PCR reaction;
  • cassette mutagenesis and mutator strains;
  • overcoming problems of codon bias;
  • alternative host expression systems - bacteria, yeasts, filamentous fungi

Summary

This chapter contains sections titled:

  • History of Biotechnology

  • Identifying Potential Biocatalysts for Chemical Synthesis

  • Molecular Biology for Improved Biocatalysts

  • Microbiology and Fermentation

  • Summary, Overview and Future

  • References