Chapter 3. Kinetic Resolutions Using Biotransformations

  1. John Whittall5 and
  2. Peter Sutton6
  1. Petra Gadler1,
  2. Kurt Faber1,
  3. Christopher K. Savile2,
  4. Romas J. Kazlauskas2,
  5. Andrew J. Carnell3 and
  6. Gary Breen4

Published Online: 15 DEC 2009

DOI: 10.1002/9780470748589.ch3

Practical Methods for Biocatalysis and Biotransformations

Practical Methods for Biocatalysis and Biotransformations

How to Cite

Gadler, P., Faber, K., Savile, C. K., Kazlauskas, R. J., Carnell, A. J. and Breen, G. (2009) Kinetic Resolutions Using Biotransformations, in Practical Methods for Biocatalysis and Biotransformations (eds J. Whittall and P. Sutton), John Wiley & Sons, Ltd, Chichester, UK. doi: 10.1002/9780470748589.ch3

Editor Information

  1. 5

    Manchester Interdisciplinary Biocentre, University of Manchester, United Kingdom

  2. 6

    GlaxoSmithKline, Synthetic Chemistry, Gunnels Wood Road, Stevenage, Hertfordshire SG1 2NY, UK

Author Information

  1. 1

    Department of Chemistry, Organic and Bioorganic Chemistry, University of Graz, Heinrichstrasse 28, 8010 Graz, Austria

  2. 2

    Department of Biochemistry, Molecular Biology & Biophysics and The Biotechnology Institute, University of Minnesota, 1479 Gortner Avenue, Saint Paul, MN 55108 USA

  3. 3

    Department of Chemistry, Robert Robinson Laboratories, University of Liverpool, Liverpool, L69 7ZD, UK

  4. 4

    GlaxoSmithKline, Synthetic Chemistry, Leigh, Tonbridge, Kent, TN11 9AN, UK

Publication History

  1. Published Online: 15 DEC 2009
  2. Published Print: 18 DEC 2009

ISBN Information

Print ISBN: 9780470519271

Online ISBN: 9780470748589



  • kinetic resolutions using biotransformations;
  • enzymatic hydrolysis of alkylsulfate esters catalysed by alkylsulfatases;
  • biocatalyst preparation;
  • microbial hydrolysis of rac-2-Octylsulfate;
  • protease-catalyzed resolutions using 3-(3-Pyridine)propionyl anchor group;
  • prochiral ketone desymmetrization using enzymes;
  • Procedure 1 - Humicola sp. Lipase BREP;
  • desymmetrization of related ketones;
  • reactions in THF using freeze-dried Amano AK PFL


This chapter contains sections titled:

  • Stereo- and Enantio-selective Hydrolysis of rac-2-Octylsulfate Using Whole Resting Cells of Pseudomonas spp.

  • Protease-catalyzed Resolutions Using the 3-(3-Pyridine)propionyl Anchor Group: p-Toluenesulfonamide

  • Desymmetrization of Prochiral Ketones Using Enzymes

  • Enzymatic Resolution of 1-Methyl-tetrahydroisoquinoline using Candida rugosa Lipase

  • References and Notes