Chapter 6. Aldolase Enzymes for Complex Synthesis

  1. John Whittall8 and
  2. Peter Sutton9
  1. William A. Greenberg1,
  2. Chi-Huey Wong1,
  3. Marielle Lemaire2,
  4. Lahssen El Blidi2,
  5. José A. Castillo3,
  6. Teodor Parella4,
  7. Tomoyuki Inoue5,
  8. Georg A. Sprenger5,
  9. Jesús Joglar3,
  10. Pere Clapés3,
  11. Franck Charmantray2,
  12. Philippe Dellis7,
  13. Virgil Hélaine2,
  14. Soth Samreth7 and
  15. Laurence Hecquet2

Published Online: 15 DEC 2009

DOI: 10.1002/9780470748589.ch6

Practical Methods for Biocatalysis and Biotransformations

Practical Methods for Biocatalysis and Biotransformations

How to Cite

Greenberg, W. A., Wong, C.-H., Lemaire, M., Blidi, L. E., Castillo, J. A., Parella, T., Inoue, T., Sprenger, G. A., Joglar, J., Clapés, P., Charmantray, F., Dellis, P., Hélaine, V., Samreth, S. and Hecquet, L. (2009) Aldolase Enzymes for Complex Synthesis, in Practical Methods for Biocatalysis and Biotransformations (eds J. Whittall and P. Sutton), John Wiley & Sons, Ltd, Chichester, UK. doi: 10.1002/9780470748589.ch6

Editor Information

  1. 8

    Manchester Interdisciplinary Biocentre, University of Manchester, United Kingdom

  2. 9

    GlaxoSmithKline, Synthetic Chemistry, Gunnels Wood Road, Stevenage, Hertfordshire SG1 2NY, UK

Author Information

  1. 1

    Department of Chemistry, The Scripps Research Institute, 10550 North Torrey Pines Rd., La Jolla, CA 92307, USA

  2. 2

    Laboratoire SEESIB, UMR 6504 CNRS, Université Blaise Pascal, 24 avenue des Landais 63177 Aubière cedex, France

  3. 3

    Biotransformation and Bioactive Molecules Group, Instituto de Quimica Avanzada de Catalun˜a, Consejo Superior de Investigaciones Cientificas, Jordi Girona 18-26, 08034 Barcelona, Spain

  4. 4

    Servei de Ressonància Magnètica Nuclear, Universitat Autònoma de Barcelona, 08193 Bellaterra, Barcelona, Spain

  5. 5

    Institute of Microbiology, University of Stuttgart, Allmandring 31, 70569 Stuttgart, Germany

  6. 7

    Fournier Pharma, 50 rue de Dijon, 21121 Daix, France

Publication History

  1. Published Online: 15 DEC 2009
  2. Published Print: 18 DEC 2009

ISBN Information

Print ISBN: 9780470519271

Online ISBN: 9780470748589



  • aldolase enzymes for complex synthesis;
  • one-step synthesis of L-fructose using rhamnulose-1-phosphate aldolase in borate buffer;
  • dihydroxyacetone phosphate (DHAP)-dependent aldolases - powerful catalysts in carbohydrate synthesis;
  • straightforward RAMA-mediated synthesis of aminocyclitols;
  • nitrocyclitol synthesis using RAMA;
  • catalysed synthesis of D-fagomine;
  • D-fagomine - stereoselectively in two chemo-enzymatic asymmetric steps using achiral DHA;
  • chemoenzymatic synthesis of 5-thio-D-xylopyranose;
  • new chemoenzymatic synthesis of 5-thio-D-xylopyranose


This chapter contains sections titled:

  • One-step Synthesis of L-Fructose Using Rhamnulose-1-phosphate Aldolase in Borate Buffer

  • Straightforward Fructose-1,6-bisphosphate Aldolase mediated Synthesis of Aminocyclitols

  • Synthesis of D-Fagomine by Aldol Addition of Dihydroxyacetone to N-Cbz-3-Aminopropanal Catalysed by D-Fructose-6-phosphate Aldolase

  • Chemoenzymatic Synthesis of 5-Thio-D-xylopyranose

  • References