Chapter 8. Synthesis of Cyanohydrins Using Hydroxynitrile Lyases

  1. John Whittall8 and
  2. Peter Sutton9
  1. Manuela Avi1,
  2. Herfried Griengl2,
  3. Chris Roberge3,
  4. Fred Fleitz3,
  5. Paul Devine3,
  6. Jan von Langermann4,
  7. Annett Mell5,
  8. Eckhard Paetzold6,
  9. Udo Kragl5 and
  10. Yasuhisa Asano7

Published Online: 15 DEC 2009

DOI: 10.1002/9780470748589.ch8

Practical Methods for Biocatalysis and Biotransformations

Practical Methods for Biocatalysis and Biotransformations

How to Cite

Avi, M., Griengl, H., Roberge, C., Fleitz, F., Devine, P., von Langermann, J., Mell, A., Paetzold, E., Kragl, U. and Asano, Y. (2009) Synthesis of Cyanohydrins Using Hydroxynitrile Lyases, in Practical Methods for Biocatalysis and Biotransformations (eds J. Whittall and P. Sutton), John Wiley & Sons, Ltd, Chichester, UK. doi: 10.1002/9780470748589.ch8

Editor Information

  1. 8

    Manchester Interdisciplinary Biocentre, University of Manchester, United Kingdom

  2. 9

    GlaxoSmithKline, Synthetic Chemistry, Gunnels Wood Road, Stevenage, Hertfordshire SG1 2NY, UK

Author Information

  1. 1

    Institute of Organic Chemistry, Graz University of Technology, Stremayrgasse 16, 8010 Graz, Austria

  2. 2

    Research Centre Applied Biocatalysis, Petersgasse 14, 8010Graz, Austria

  3. 3

    Process Research, Merck Research Laboratories, Merck & Co. Inc. Rahway, NJ, USA

  4. 4

    Max-Planck-Institut für Dynamik komplexer technischer Systeme, Physikalisch-Chemische Grundlagen der Prozesstechnik, Sandtorstr.1 D-39106 Magdeburg, Germany

  5. 5

    Institut für Chemie, Universität Rostock, Albert-Einstein-Str. 3a, 18059 Rostock, Germany

  6. 6

    Leibniz-Institut für Katalyse, A.-Einstein-Str. 29a, 18059 Rostock, Germany

  7. 7

    Biotechnology Research Center and Department of Biotechnology, Toyama Prefectural University, 5180 Kurokawa, Imizu, Toyama 939-0398, Japan

Publication History

  1. Published Online: 15 DEC 2009
  2. Published Print: 18 DEC 2009

ISBN Information

Print ISBN: 9780470519271

Online ISBN: 9780470748589



  • synthesis of cyanohydrins using hydroxynitrile lyases;
  • hydroxynitrile lyase (HNL)-catalysed cyanohydrin reaction – method in synthesizing enantiopure-hydroxy nitriles;
  • chemoenzymatic synthesis of (S)-2-hydroxy-2-methylbutyric acid;
  • hydroxynitrile lyase (HNL) enzymes - effective biocatalysts;
  • enzymatic (S)-selective substituted phenylacetone cyanohydrin synthesis;
  • M. esculenta-catalysed synthesis of acetophenone cyanohydrins;
  • cyanohydrin formation from pyridine-3-carboxaldehyde;
  • new hydroxynitrile lyase (HNL) - isolated from seeds of Japanese apricot (Prunus mume)


This chapter contains sections titled:

  • Synthesis of (S)-2-Hydroxy-2-methylbutyric Acid by a Chemoenzymatic Methodology

  • (S)-Selective Cyanohydrin Formation from Aromatic Ketones Using Hydroxynitrile Lyases

  • Hydroxynitrile-lyase-catalysed Synthesis of Enantiopure (S)-Acetophenone Cyanohydrins

  • (R)- and (S)-Cyanohydrin Formation from Pyridine-3-carboxaldehyde Using CLEATM-immobilized Hydroxynitrile Lyases

  • A New (R)-Hydroxynitrile Lyase from Prunus mume for Asymmetric Synthesis of Cyanohydrins

  • References