Chapter 1. Measurement of Ligand–G Protein-Coupled Receptor Interactions

  1. David R. Poyner2 and
  2. Mark Wheatley3
  1. Katie Leach,
  2. Celine Valant,
  3. Patrick M. Sexton and
  4. Arthur Christopoulos

Published Online: 25 JAN 2010

DOI: 10.1002/9780470749210.ch1

G Protein-Coupled Receptors: Essential Methods

G Protein-Coupled Receptors: Essential Methods

How to Cite

Leach, K., Valant, C., Sexton, P. M. and Christopoulos, A. (2010) Measurement of Ligand–G Protein-Coupled Receptor Interactions, in G Protein-Coupled Receptors: Essential Methods (eds D. R. Poyner and M. Wheatley), Wiley-Blackwell, Oxford, UK. doi: 10.1002/9780470749210.ch1

Editor Information

  1. 2

    Pharmaceutical and Biological Sciences, School of Life and Health Sciences, Aston University, Birmingham B4 7ET, UK

  2. 3

    School of Biosciences, Birmingham University, Birmingham B15 2TT, UK

Author Information

  1. Drug Discovery Biology Laboratory, Department of Pharmacology, Monash University, Clayton 3800, Australia

Publication History

  1. Published Online: 25 JAN 2010
  2. Published Print: 22 JAN 2010

ISBN Information

Print ISBN: 9780470749142

Online ISBN: 9780470749210

SEARCH

Keywords:

  • measurement of ligand–G protein-coupled receptor interactions;
  • ligand–receptor interactions and law of mass action;
  • Hill–Langmuir binding isotherm;
  • ligand–G protein-coupled receptor (GPCR) interactions;
  • allosteric ligands;
  • radioligand binding assays;
  • Protocol 1.2 saturation binding assays;
  • Protocol 1.3 competition binding assays;
  • Protocol 1.5 radioligand binding kinetics measurement;
  • radioligand dissociation kinetics in allosteric modulator presence

Summary

This chapter contains sections titled:

  • Introduction

  • Methods and approaches

  • References