Chapter 10. Identification and Analysis of GPCR Phosphorylation

  1. David R. Poyner2 and
  2. Mark Wheatley3
  1. Kok Choi Kong,
  2. Sharad C. Mistry and
  3. Andrew B. Tobin

Published Online: 25 JAN 2010

DOI: 10.1002/9780470749210.ch10

G Protein-Coupled Receptors: Essential Methods

G Protein-Coupled Receptors: Essential Methods

How to Cite

Kong, K. C., Mistry, S. C. and Tobin, A. B. (2010) Identification and Analysis of GPCR Phosphorylation, in G Protein-Coupled Receptors: Essential Methods (eds D. R. Poyner and M. Wheatley), Wiley-Blackwell, Oxford, UK. doi: 10.1002/9780470749210.ch10

Editor Information

  1. 2

    Pharmaceutical and Biological Sciences, School of Life and Health Sciences, Aston University, Birmingham B4 7ET, UK

  2. 3

    School of Biosciences, Birmingham University, Birmingham B15 2TT, UK

Author Information

  1. Department of Cell Physiology and Pharmacology, University of Leicester, Hodgkin Building, Lancaster Road, Leicester LE1 9HN, UK

Publication History

  1. Published Online: 25 JAN 2010
  2. Published Print: 22 JAN 2010

ISBN Information

Print ISBN: 9780470749142

Online ISBN: 9780470749210

SEARCH

Keywords:

  • GPCR phosphorylation - identification and analysis;
  • G protein-coupled receptor kinase (GRK) family;
  • phosphorylation studies of GPCRs - requiring purification of receptors;
  • anti-receptor antibodies for use in immunoprecipitation studies;
  • sodium dodecyl sulfate (SDS)–polyacrylamide gel electrophoresis (PAGE) gels;
  • Protocol 10.1 preparation of anti-receptor antibodies;
  • biotinylation immunoprecipitation;
  • resolving immunoprecipitated receptor using SDS–PAGE;
  • Edman degradation of hot phosphopeptides isolated from phosphopeptide maps;
  • PAA analysis

Summary

This chapter contains sections titled:

  • Introduction

  • Methods

  • Acknowledgements

  • References