Chapter 11. Measurement and Visualization of G Protein-coupled Receptor Trafficking by Enzyme-linked Immunosorbent Assay and Immunofluorescence

  1. David R. Poyner2 and
  2. Mark Wheatley3
  1. Stuart J. Mundell,
  2. Shaista P. Nisar and
  3. Eamonn Kelly

Published Online: 25 JAN 2010

DOI: 10.1002/9780470749210.ch11

G Protein-Coupled Receptors: Essential Methods

G Protein-Coupled Receptors: Essential Methods

How to Cite

Mundell, S. J., Nisar, S. P. and Kelly, E. (2010) Measurement and Visualization of G Protein-coupled Receptor Trafficking by Enzyme-linked Immunosorbent Assay and Immunofluorescence, in G Protein-Coupled Receptors: Essential Methods (eds D. R. Poyner and M. Wheatley), Wiley-Blackwell, Oxford, UK. doi: 10.1002/9780470749210.ch11

Editor Information

  1. 2

    Pharmaceutical and Biological Sciences, School of Life and Health Sciences, Aston University, Birmingham B4 7ET, UK

  2. 3

    School of Biosciences, Birmingham University, Birmingham B15 2TT, UK

Author Information

  1. Department of Physiology & Pharmacology, University of Bristol, Medical Sciences Building, University Walk, Bristol BS8 1TD, UK

Publication History

  1. Published Online: 25 JAN 2010
  2. Published Print: 22 JAN 2010

ISBN Information

Print ISBN: 9780470749142

Online ISBN: 9780470749210

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Keywords:

  • measurement and visualization of G protein-coupled receptor trafficking;
  • internalization of G protein-coupled receptors (GPCRs) - major mechanism for signalling level regulation;
  • reliable measure of GPCR internalization in response to agonist;
  • loss of surface GPCR by ELISA;
  • ELISA, expression of epitope-tagged GPCR in cell line of choice;
  • Protocol 11.1 measurement of surface receptor loss;
  • receptor recycling measurement;
  • cell preparation for receptor trafficking visualization;
  • Protocol 11.3 preparation of cells for visualization of internalized GPCRs by microscopy;
  • poor triplicates in ELISA

Summary

This chapter contains sections titled:

  • Introduction

  • Methods and approaches

  • Troubleshooting

  • References