Chapter 12. Substituted Cysteine Accessibility Method (SCAM)

  1. David R. Poyner3 and
  2. Mark Wheatley4
  1. George Liapakis1 and
  2. Jonathan A. Javitch2

Published Online: 25 JAN 2010

DOI: 10.1002/9780470749210.ch12

G Protein-Coupled Receptors: Essential Methods

G Protein-Coupled Receptors: Essential Methods

How to Cite

Liapakis, G. and Javitch, J. A. (2010) Substituted Cysteine Accessibility Method (SCAM), in G Protein-Coupled Receptors: Essential Methods (eds D. R. Poyner and M. Wheatley), Wiley-Blackwell, Oxford, UK. doi: 10.1002/9780470749210.ch12

Editor Information

  1. 3

    Pharmaceutical and Biological Sciences, School of Life and Health Sciences, Aston University, Birmingham B4 7ET, UK

  2. 4

    School of Biosciences, Birmingham University, Birmingham B15 2TT, UK

Author Information

  1. 1

    Faculty of Medicine, University of Crete, Voutes, Heraklion 71003, Crete, Greece

  2. 2

    Center for Molecular Recognition, Columbia University, P&S 11-401, Box 7, 630 West 168th Street, New York, NY 10032, USA

Publication History

  1. Published Online: 25 JAN 2010
  2. Published Print: 22 JAN 2010

ISBN Information

Print ISBN: 9780470749142

Online ISBN: 9780470749210

SEARCH

Keywords:

  • substituted cysteine accessibility method (SCAM);
  • G protein-coupled receptor (GPCR) superfamily;
  • substituted Cys accessibility method and its assumptions;
  • reaction of MTS reagent with Cys sulfhydryl at water-accessible surface of receptor;
  • endogenous Cys;
  • Protocol 12.1 transfection of HEK 293 cells;
  • human embryonic kidney cells (HEK 293) in DMEM–F12;
  • Protocol 12.3 ligand binding;
  • Protocol 12.5 protecting ligand binding reaction;
  • conformational changes associated with receptor activation

Summary

This chapter contains sections titled:

  • Introduction

  • Methods and approaches

  • Troubleshooting

  • References