Chapter 2. Second Messenger Assays for G Protein-Coupled Receptors: cAMP, Ca2+, Inositol Phosphates, ERK1/2

  1. David R. Poyner2 and
  2. Mark Wheatley3
  1. Karen J. Gregory,
  2. Patrick M. Sexton,
  3. Arthur Christopoulos and
  4. Caroline A. Hick

Published Online: 25 JAN 2010

DOI: 10.1002/9780470749210.ch2

G Protein-Coupled Receptors: Essential Methods

G Protein-Coupled Receptors: Essential Methods

How to Cite

Gregory, K. J., Sexton, P. M., Christopoulos, A. and Hick, C. A. (2010) Second Messenger Assays for G Protein-Coupled Receptors: cAMP, Ca2+, Inositol Phosphates, ERK1/2, in G Protein-Coupled Receptors: Essential Methods (eds D. R. Poyner and M. Wheatley), Wiley-Blackwell, Oxford, UK. doi: 10.1002/9780470749210.ch2

Editor Information

  1. 2

    Pharmaceutical and Biological Sciences, School of Life and Health Sciences, Aston University, Birmingham B4 7ET, UK

  2. 3

    School of Biosciences, Birmingham University, Birmingham B15 2TT, UK

Author Information

  1. Drug Discovery Biology Laboratory, Department of Pharmacology, Monash University, Clayton 3800, Australia

Publication History

  1. Published Online: 25 JAN 2010
  2. Published Print: 22 JAN 2010

ISBN Information

Print ISBN: 9780470749142

Online ISBN: 9780470749210

SEARCH

Keywords:

  • second messenger assays for G protein-coupled receptors - cAMP, Ca2+, Inositol Phosphates, ERK1/2;
  • G protein-coupled receptors (GPCRs);
  • major intracellular signalling pathways from G protein coupling;
  • cAMP, tightly regulated signalling molecule in extracellular signal transduction;
  • semi-automated filtration-based anion-exchange chromatography approaches;
  • calcium ions - widely assayed intracellular messengers;
  • Protocol 2.3 fluo-4 calcium assay;
  • mitogen-activated protein kinases;
  • troubleshooting;
  • successful assay - cells expressing GPCR of interest

Summary

This chapter contains sections titled:

  • Introduction

  • Methods and approaches

  • Troubleshooting

  • References