Chapter 3. Use of the [35S]GTPγS Binding Assay to Determine Ligand Efficacy at G Protein-Coupled Receptors

  1. David R. Poyner2 and
  2. Mark Wheatley3
  1. Elodie Kara and
  2. Philip G. Strange

Published Online: 25 JAN 2010

DOI: 10.1002/9780470749210.ch3

G Protein-Coupled Receptors: Essential Methods

G Protein-Coupled Receptors: Essential Methods

How to Cite

Kara, E. and Strange, P. G. (2010) Use of the [35S]GTPγS Binding Assay to Determine Ligand Efficacy at G Protein-Coupled Receptors, in G Protein-Coupled Receptors: Essential Methods (eds D. R. Poyner and M. Wheatley), Wiley-Blackwell, Oxford, UK. doi: 10.1002/9780470749210.ch3

Editor Information

  1. 2

    Pharmaceutical and Biological Sciences, School of Life and Health Sciences, Aston University, Birmingham B4 7ET, UK

  2. 3

    School of Biosciences, Birmingham University, Birmingham B15 2TT, UK

Author Information

  1. School of Pharmacy, University of Reading, PO Box 228, Whiteknights, Reading RG6 6AJ, UK

Publication History

  1. Published Online: 25 JAN 2010
  2. Published Print: 22 JAN 2010

ISBN Information

Print ISBN: 9780470749142

Online ISBN: 9780470749210

SEARCH

Keywords:

  • [35S]GTPγS binding assay - determining ligand efficacy at G protein-coupled receptors;
  • methods for assessing efficacy - for agonists for G protein-coupled receptors (GPCRs);
  • understanding and quantifying drug efficacy;
  • in vitro systems to measure agonist efficacy;
  • Chinese hamster ovary (CHO) cells - expressing recombinant GPCR;
  • Protocol 3.2 determination of concentration of GDP in [35S]GTPγS binding assay;
  • Protocol 3.3 data analysis of GDP requirements;
  • other assays for efficacy at GPCRs;
  • [35S]GTPγS binding assay - under conditions for GPCRs coupled to Gi/o proteins;
  • BSA in assays for preventing ligand loss

Summary

This chapter contains sections titled:

  • Introduction

  • Methods and approaches

  • Troubleshooting

  • Acknowledgements

  • References