Chapter 6. Monitoring GPCR–Protein Complexes Using Bioluminescence Resonance Energy Transfer

  1. David R. Poyner2 and
  2. Mark Wheatley3
  1. Werner C. Jaeger,
  2. Kevin D. G. Pfleger and
  3. Karin A. Eidne

Published Online: 25 JAN 2010

DOI: 10.1002/9780470749210.ch6

G Protein-Coupled Receptors: Essential Methods

G Protein-Coupled Receptors: Essential Methods

How to Cite

Jaeger, W. C., Pfleger, K. D. G. and Eidne, K. A. (2010) Monitoring GPCR–Protein Complexes Using Bioluminescence Resonance Energy Transfer, in G Protein-Coupled Receptors: Essential Methods (eds D. R. Poyner and M. Wheatley), Wiley-Blackwell, Oxford, UK. doi: 10.1002/9780470749210.ch6

Editor Information

  1. 2

    Pharmaceutical and Biological Sciences, School of Life and Health Sciences, Aston University, Birmingham B4 7ET, UK

  2. 3

    School of Biosciences, Birmingham University, Birmingham B15 2TT, UK

Author Information

  1. Laboratory for Molecular Endocrinology – GPCRs, WAIMR and UWA Centre for Medical Research, QEII Medical Centre, Nedlands, Perth, Western Australia 6009, Australia

Publication History

  1. Published Online: 25 JAN 2010
  2. Published Print: 22 JAN 2010

ISBN Information

Print ISBN: 9780470749142

Online ISBN: 9780470749210

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Keywords:

  • monitoring GPCR–protein complexes using bioluminescence resonance energy transfer;
  • formation of multiple protein complexes - activation life cycle of G protein-coupled receptors (GPCRs);
  • Fourier transform infrared spectroscopy (FTIR) and biological macromolecule structure;
  • conventional biochemical methods - at detecting protein–protein interactions;
  • BRET principles;
  • measuring GPCR–protein interactions using BRET;
  • BRET expression systems and fusion protein transfection;
  • choice of BRET method and procedure;
  • low or no protein expression;
  • low or no BRET signal

Summary

This chapter contains sections titled:

  • Introduction

  • Methods and approaches

  • Troubleshooting

  • References