Chapter 9. Use of Fluorescence Correlation Spectroscopy to Study the Diffusion of G Protein-coupled Receptors

  1. David R. Poyner2 and
  2. Mark Wheatley3
  1. Stephen J. Briddon,
  2. Jonathan A. Hern and
  3. Stephen J. Hill

Published Online: 25 JAN 2010

DOI: 10.1002/9780470749210.ch9

G Protein-Coupled Receptors: Essential Methods

G Protein-Coupled Receptors: Essential Methods

How to Cite

Briddon, S. J., Hern, J. A. and Hill, S. J. (2010) Use of Fluorescence Correlation Spectroscopy to Study the Diffusion of G Protein-coupled Receptors, in G Protein-Coupled Receptors: Essential Methods (eds D. R. Poyner and M. Wheatley), Wiley-Blackwell, Oxford, UK. doi: 10.1002/9780470749210.ch9

Editor Information

  1. 2

    Pharmaceutical and Biological Sciences, School of Life and Health Sciences, Aston University, Birmingham B4 7ET, UK

  2. 3

    School of Biosciences, Birmingham University, Birmingham B15 2TT, UK

Author Information

  1. Institute of Cell Signalling, School of Biomedical Sciences, University of Nottingham, Nottingham NG7 2UH, UK

Publication History

  1. Published Online: 25 JAN 2010
  2. Published Print: 22 JAN 2010

ISBN Information

Print ISBN: 9780470749142

Online ISBN: 9780470749210

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Keywords:

  • fluorescence correlation spectroscopy - studying diffusion of G protein-coupled receptors;
  • fluorescence recovery after photobleaching (FRAP);
  • fluorescence correlation spectroscopy (FCS);
  • principles of FCS;
  • basic components of a single-channel FCS microscope using continuous-wave laser excitation;
  • routine calibration of FCS microscope;
  • measuring GPCR diffusion in cell membrane;
  • measuring diffusion of ligand-occupied GPCR;
  • diffusion times and particle numbers - from autocorrelation data using nonlinear curve-fitting techniques;
  • change in diffusion time - proportional to cube root of mass difference

Summary

This chapter contains sections titled:

  • Introduction

  • Methods and approaches

  • Troubleshooting

  • References