Chapter 10. Tracking Intracellular Polymer Localization Via Fluorescence Microscopy

  1. Volkmar Weissig2 and
  2. Gerard G. M. D'Souza3
  1. Simon C. W. Richardson

Published Online: 25 AUG 2010

DOI: 10.1002/9780470875780.ch10

Organelle-Specific Pharmaceutical Nanotechnology

Organelle-Specific Pharmaceutical Nanotechnology

How to Cite

Richardson, S. C. W. (2010) Tracking Intracellular Polymer Localization Via Fluorescence Microscopy, in Organelle-Specific Pharmaceutical Nanotechnology (eds V. Weissig and G. G. M. D'Souza), John Wiley & Sons, Inc., Hoboken, NJ, USA. doi: 10.1002/9780470875780.ch10

Editor Information

  1. 2

    Department of Pharmaceutical Sciences, Midwestern University College of Pharmacy, Glendale, Arizona, USA

  2. 3

    Department of Pharmaceutical Sciences, Massachusetts College of Pharmacy and Health Sciences, Boston, Massachusetts, USA

Author Information

  1. University of Greenwich School of Science, Kent, England, UK

Publication History

  1. Published Online: 25 AUG 2010
  2. Published Print: 11 OCT 2010

ISBN Information

Print ISBN: 9780470631652

Online ISBN: 9780470875780



  • tracking intracellular polymer localization - via fluorescence microscopy;
  • LAMPs, highly glycosylated integral membrane proteins - C-terminal cytosolic sorting signal, directing proteins to late endocytic structures;
  • sample preparation - for poorly fixable material


This chapter contains sections titled:

  • An Introduction to the Study of the Endocytic and Secretory Pathways Using Fluorescent Microscopy

  • Endocytic and Secretory Organelles: Function and Markers

  • Fixation

  • Sample Preparation for Poorly Fixable Material

  • References