8. Automation of Immunoassays

  1. Guomin Shan
  1. Michele Yarnall

Published Online: 30 NOV 2010

DOI: 10.1002/9780470909935.ch8

Immunoassays in Agricultural Biotechnology

Immunoassays in Agricultural Biotechnology

How to Cite

Yarnall, M. (2011) Automation of Immunoassays, in Immunoassays in Agricultural Biotechnology (ed G. Shan), John Wiley & Sons, Inc., Hoboken, NJ, USA. doi: 10.1002/9780470909935.ch8

Editor Information

  1. Dow AgroSciences LLC, Indianapolis, Indiana, USA

Author Information

  1. Syngenta Biotechnology, Inc., Research Triangle Park, North Carolina, USA

Publication History

  1. Published Online: 30 NOV 2010
  2. Published Print: 24 JAN 2011

ISBN Information

Print ISBN: 9780470289525

Online ISBN: 9780470909935



  • immunoassay automation, in pharmaceutical and clinical diagnostic fields;
  • automated systems, in pharmaceutical and clinical diagnostic fields - not suited for testing plant extracts due to particulate nature of extract;
  • bottlenecks, in automating process of testing plant samples - ELISA format, transfer of plant extract in four 96-well blocks to one 384-well microtiter plate


This chapter contains sections titled:

  • Introduction to Immunoassay Automation

  • Increasing Throughput by Automation

  • Tissue Collection

  • Sample Processing

  • Sample Transfer

  • Reagent Additions

  • Washing and Reading Steps

  • Analysis

  • Achieving Full Automation by Connecting the Automated Steps

  • Duplexing

  • Future Technologies

  • Summary

  • References