15. Confirmation of a Mutation by Multiple Molecular Approaches

  1. Weikuan Gu2 and
  2. Yongjun Wang3
  1. Hector Martinez-Valdez and
  2. Blanca Ortiz-Quintero

Published Online: 10 FEB 2012

DOI: 10.1002/9780470933947.ch15

Gene Discovery for Disease Models

Gene Discovery for Disease Models

How to Cite

Martinez-Valdez, H. and Ortiz-Quintero, B. (2011) Confirmation of a Mutation by Multiple Molecular Approaches, in Gene Discovery for Disease Models (eds W. Gu and Y. Wang), John Wiley & Sons, Inc., Hoboken, NJ, USA. doi: 10.1002/9780470933947.ch15

Editor Information

  1. 2

    Department of Orthopedic Surgery—Campbell Clinic, University of Tennessee Health Science Center, Memphis, TN, USA

  2. 3

    Beijing Tiantan Hospital, Capital Medical University, Beijing, China

Author Information

  1. Department of Immunology, The University of Texas M. D. Anderson Cancer Center, Houston, TX, USA

Publication History

  1. Published Online: 10 FEB 2012
  2. Published Print: 21 MAR 2011

ISBN Information

Print ISBN: 9780470499467

Online ISBN: 9780470933947

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Keywords:

  • mutation confirmation, by multiple molecular approaches - gene rearrangement, and alternative polyadenylation impact on gene expression control;
  • MRNA expression by real-time PCR - quantitative real-time PCR, center-stage attention;
  • genetically engineered animals, in vitro - key gene functions and pathway involvement, crucial for cellular operations

Summary

This chapter contains sections titled:

  • Introduction

  • mRNA Expression by Real-Time PCR

  • DNA Sequencing

  • In Situ Hybridization

  • Expression at the Protein Level

  • Genetically Engineered Animals

  • Concluding Remarks

  • Acknowledgments

  • References